Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,840,838 T→G 6.7% A126A (GCT→GCG ynjD → predicted transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,840,8380TG 6.7% 138.0 / 5.6 60A126A (GCT→GCGynjDpredicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (26/29);  new base G (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

TAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTC  >  W3110S.gb/1840775‑1840907
                                                               |                                                                     
tAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCAt                                                                 >  1:862658/1‑70 (MQ=255)
tAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCAt                                                                 >  1:2811114/1‑70 (MQ=255)
tAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCAt                                                                 >  1:3950533/1‑70 (MQ=255)
 aaaGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATc                                                                <  1:156320/70‑1 (MQ=255)
  aaGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCa                                                               <  1:187410/70‑1 (MQ=255)
    gggAATGCCCGACGTAATGCCGTGAATGATGCTCTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATGAGGa                                                            <  1:4050209/71‑1 (MQ=255)
    gggAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGa                                                            <  1:488288/71‑1 (MQ=255)
    gggAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGa                                                            <  1:129017/71‑1 (MQ=255)
     ggAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGAt                                                           <  1:4184273/71‑1 (MQ=255)
     ggAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGAt                                                           <  1:4179271/71‑1 (MQ=255)
         tGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGa                                                            <  1:1475094/66‑1 (MQ=255)
         tGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTg                                                       >  1:1258922/1‑71 (MQ=255)
         tGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGACGGAGCGTTCCATCAGGATCCTg                                                       <  1:2524566/71‑1 (MQ=255)
             cGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATc                                                                >  1:3710256/1‑58 (MQ=255)
                 gTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTg                                               <  1:2832804/71‑1 (MQ=255)
                   aaTGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCtt                                                                      >  1:3168093/1‑46 (MQ=255)
                    aTGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCt                                            >  1:2929056/1‑71 (MQ=255)
                     tGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTg                                           <  1:2574727/71‑1 (MQ=255)
                      gCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATcc                                                         >  1:2189524/1‑56 (MQ=255)
                       ccGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGt                                              >  1:3372203/1‑66 (MQ=255)
                       ccGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTgg                                          <  1:3997710/70‑1 (MQ=255)
                          tGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCtt                                                                      <  1:4087056/39‑1 (MQ=255)
                          tGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCtt                                                                      <  1:992952/39‑1 (MQ=255)
                            aaTGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTc                                             >  1:225718/1‑62 (MQ=255)
                            aaTGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGt                                      >  1:3757154/1‑69 (MQ=255)
                            aaTGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGt                                      >  1:1852225/1‑69 (MQ=255)
                            aaTGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTCCACTTTGTCTGGCGGTCag                                   >  1:1299956/1‑71 (MQ=255)
                                atgCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCagc                                  >  1:4163252/1‑69 (MQ=255)
                                atgCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCagc                                  >  1:3148467/1‑69 (MQ=255)
                                atgCACTTGAGCGTTCAGGCCTTGACGGAGCGTTCCATCAGGATCCTg                                                       <  1:3273292/48‑1 (MQ=38)
                                  gCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCagcgagc                              >  1:3531567/1‑71 (MQ=255)
                                   cACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCa                                                               >  1:3816311/1‑37 (MQ=255)
                                    aCTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCATGATCCTGCCACTTTGTCTGGctg                                       >  1:1177429/1‑58 (MQ=255)
                                    aCTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCagcgagc                              <  1:473431/69‑1 (MQ=255)
                                     cTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg                           <  1:3328974/71‑1 (MQ=255)
                                     cTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg                           <  1:2689036/71‑1 (MQ=255)
                                      ttGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACttt                                                <  1:1778686/49‑1 (MQ=255)
                                      ttGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCt                                            <  1:2813775/53‑1 (MQ=255)
                                       tGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg                           <  1:2636656/69‑1 (MQ=255)
                                        gAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATcc                                                         >  1:3639246/1‑38 (MQ=255)
                                        gAGCGTTCAGGCCTTGAGGGAGCATTCCATCAGGATc                                                          >  1:542310/1‑37 (MQ=255)
                                         aGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGt                        <  1:200025/70‑1 (MQ=255)
                                          gCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTg                      <  1:718419/71‑1 (MQ=255)
                                           cGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGc                     <  1:1220275/71‑1 (MQ=255)
                                               cAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCa                                    >  1:1810189/1‑52 (MQ=255)
                                               cAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGTGCGTTGctct                  <  1:3399898/70‑1 (MQ=255)
                                               cAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTcta                 >  1:2569302/1‑71 (MQ=255)
                                               cAGGCCTTGACGGAGCGTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGctctg                 >  1:2543815/1‑70 (MQ=255)
                                               cAGCCCTTGACGGAGCGTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGctctg                 >  1:4226075/1‑70 (MQ=255)
                                                aGGCCTTGAGGGAGCTTTCCATGAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcgca                         >  1:1119422/1‑61 (MQ=255)
                                                aGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGcgcgcg                         >  1:1862918/1‑62 (MQ=255)
                                                aGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTctac                <  1:3585663/71‑1 (MQ=255)
                                                aGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTcta                 <  1:2254082/70‑1 (MQ=255)
                                                aGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTcta                 <  1:2457905/70‑1 (MQ=255)
                                                 ggCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTctact               <  1:2332175/71‑1 (MQ=255)
                                                  gCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTctacta              >  1:4264094/1‑71 (MQ=255)
                                                    cTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTg                                               <  1:1301278/36‑1 (MQ=255)
                                                       gAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTcta                 >  1:3035102/1‑63 (MQ=255)
                                                       gAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACgcgc         >  1:720978/1‑71 (MQ=255)
                                                          ggAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg                           <  1:3452059/50‑1 (MQ=255)
                                                          ggAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg                           <  1:129155/50‑1 (MQ=255)
                                                            aGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTtc    >  1:460826/1‑71 (MQ=255)
                                                              cTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTtctc  <  1:1786261/71‑1 (MQ=255)
                                                               tttCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTg                      <  1:167054/50‑1 (MQ=255)
                                                               |                                                                     
TAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTC  >  W3110S.gb/1840775‑1840907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: