Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,840,838 | T→G | 6.7% | A126A (GCT→GCG) | ynjD → | predicted transporter subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,840,838 | 0 | T | G | 6.7% | 138.0 / 5.6 | 60 | A126A (GCT→GCG) | ynjD | predicted transporter subunit |
Reads supporting (aligned to +/- strand): ref base T (26/29); new base G (2/2); total (29/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 |
TAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTC > W3110S.gb/1840775‑1840907 | tAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCAt > 1:862658/1‑70 (MQ=255) tAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCAt > 1:2811114/1‑70 (MQ=255) tAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCAt > 1:3950533/1‑70 (MQ=255) aaaGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATc < 1:156320/70‑1 (MQ=255) aaGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCa < 1:187410/70‑1 (MQ=255) gggAATGCCCGACGTAATGCCGTGAATGATGCTCTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATGAGGa < 1:4050209/71‑1 (MQ=255) gggAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGa < 1:488288/71‑1 (MQ=255) gggAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGa < 1:129017/71‑1 (MQ=255) ggAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGAt < 1:4184273/71‑1 (MQ=255) ggAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGAt < 1:4179271/71‑1 (MQ=255) tGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGa < 1:1475094/66‑1 (MQ=255) tGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTg > 1:1258922/1‑71 (MQ=255) tGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGACGGAGCGTTCCATCAGGATCCTg < 1:2524566/71‑1 (MQ=255) cGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATc > 1:3710256/1‑58 (MQ=255) gTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTg < 1:2832804/71‑1 (MQ=255) aaTGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCtt > 1:3168093/1‑46 (MQ=255) aTGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCt > 1:2929056/1‑71 (MQ=255) tGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTg < 1:2574727/71‑1 (MQ=255) gCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATcc > 1:2189524/1‑56 (MQ=255) ccGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGt > 1:3372203/1‑66 (MQ=255) ccGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTgg < 1:3997710/70‑1 (MQ=255) tGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCtt < 1:4087056/39‑1 (MQ=255) tGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCtt < 1:992952/39‑1 (MQ=255) aaTGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTc > 1:225718/1‑62 (MQ=255) aaTGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGt > 1:3757154/1‑69 (MQ=255) aaTGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGt > 1:1852225/1‑69 (MQ=255) aaTGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCT‑CCACTTTGTCTGGCGGTCag > 1:1299956/1‑71 (MQ=255) atgCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCagc > 1:4163252/1‑69 (MQ=255) atgCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCagc > 1:3148467/1‑69 (MQ=255) atgCACTTGAGCGTTCAGGCCTTGACGGAGCGTTCCATCAGGATCCTg < 1:3273292/48‑1 (MQ=38) gCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCagcgagc > 1:3531567/1‑71 (MQ=255) cACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCa > 1:3816311/1‑37 (MQ=255) aCTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCATGATCCTGCCACTTTGTCTGGctg > 1:1177429/1‑58 (MQ=255) aCTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCagcgagc < 1:473431/69‑1 (MQ=255) cTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg < 1:3328974/71‑1 (MQ=255) cTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg < 1:2689036/71‑1 (MQ=255) ttGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACttt < 1:1778686/49‑1 (MQ=255) ttGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCt < 1:2813775/53‑1 (MQ=255) tGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg < 1:2636656/69‑1 (MQ=255) gAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATcc > 1:3639246/1‑38 (MQ=255) gAGCGTTCAGGCCTTGAGGGAGCATTCCATCAGGATc > 1:542310/1‑37 (MQ=255) aGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGt < 1:200025/70‑1 (MQ=255) gCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTg < 1:718419/71‑1 (MQ=255) cGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGc < 1:1220275/71‑1 (MQ=255) cAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCa > 1:1810189/1‑52 (MQ=255) cAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGTGCGTTGctct < 1:3399898/70‑1 (MQ=255) cAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTcta > 1:2569302/1‑71 (MQ=255) cAGGCCTTGACGGAGCGTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGctctg > 1:2543815/1‑70 (MQ=255) cAGCCCTTGACGGAGCGTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGctctg > 1:4226075/1‑70 (MQ=255) aGGCCTTGAGGGAGCTTTCCATGAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcgca > 1:1119422/1‑61 (MQ=255) aGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGcgcgcg > 1:1862918/1‑62 (MQ=255) aGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTctac < 1:3585663/71‑1 (MQ=255) aGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTcta < 1:2254082/70‑1 (MQ=255) aGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTcta < 1:2457905/70‑1 (MQ=255) ggCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTctact < 1:2332175/71‑1 (MQ=255) gCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTctacta > 1:4264094/1‑71 (MQ=255) cTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTg < 1:1301278/36‑1 (MQ=255) gAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTcta > 1:3035102/1‑63 (MQ=255) gAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACgcgc > 1:720978/1‑71 (MQ=255) ggAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg < 1:3452059/50‑1 (MQ=255) ggAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAgcgcg < 1:129155/50‑1 (MQ=255) aGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTtc > 1:460826/1‑71 (MQ=255) cTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTtctc < 1:1786261/71‑1 (MQ=255) tttCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTg < 1:167054/50‑1 (MQ=255) | TAAAGGGAATGCCCGACGTAATGCCGTGAATGATGCACTTGAGCGTTCAGGCCTTGAGGGAGCTTTCCATCAGGATCCTGCCACTTTGTCTGGCGGTCAGCGAGCGCGCGTTGCTCTACTACGCGCCCTTCTC > W3110S.gb/1840775‑1840907 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |