Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,866,685	G→A	6.0%	S222S (AGC→AGT)	yeaE ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,866,685	0	G	A	6.0%	205.5 / 2.2	67	S222S (AGC→AGT)	yeaE	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (39/23);  new base A (2/2);  total (42/25)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCC  >  W3110S.gb/1866617‑1866753                                                                     |                                                                     gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTTATCACCCACGCCAACAATACTTGTGCCgcgc                                                                     <  1:336304/70‑1 (MQ=255) gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCt                                                                    >  1:273951/1‑71 (MQ=255) gTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCt                                                                    >  1:3951257/1‑71 (MQ=255)    gCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGa                                                                  >  1:947696/1‑70 (MQ=255)      cGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGatat                                                               >  1:3486816/1‑71 (MQ=255)        cTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATtg                                                             >  1:4009807/1‑71 (MQ=255)          tttGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATtgt                                                            >  1:2674728/1‑70 (MQ=255)           ttGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATtgt                                                            >  1:3160091/1‑69 (MQ=255)            tGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAg                                                         >  1:1907934/1‑71 (MQ=255)                   gCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtgtg                                                   >  1:2561331/1‑70 (MQ=255)                   gCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCAtgtg                                                   >  1:2743780/1‑70 (MQ=255)                   gCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGc                                                  <  1:4120689/71‑1 (MQ=255)                    ccATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGa                                                                  >  1:4281382/1‑54 (MQ=255)                       tCACACCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAAtt                                              <  1:2754849/71‑1 (MQ=255)                       tCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt                                              <  1:3722316/71‑1 (MQ=255)                       tCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAtt                                              <  1:3001095/71‑1 (MQ=255)                        cacaCCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAAttt                                             >  1:1451467/1‑71 (MQ=255)                         acacCCTGATGGCTGATTACCCCCGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTc                                            >  1:3965224/1‑71 (MQ=255)                         acacCCTGATGGCTGATTACCCACGCCAACAATACTTGTGCCGCACTGATATTGTGAGCATGTGCAATTTc                                            >  1:3263572/1‑71 (MQ=255)                         acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTc                                            <  1:485009/71‑1 (MQ=255)                         acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTc                                            >  1:4184783/1‑71 (MQ=255)                         acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTc                                            >  1:1959469/1‑71 (MQ=255)                         acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTc                                            >  1:3217299/1‑71 (MQ=255)                         acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTc                                            >  1:1590204/1‑71 (MQ=255)                         acacCCTGATGACTGATCACCCACGCCAACAATACTTGTGC‑‑CGCTGATATTGTGAGCATGTGCAATTTCGt                                          >  1:4026980/1‑71 (MQ=255)                         acacCCTGATGACTGATCACCCACGCCAACAAAACTTGTGCCGCGCTGATATTGTGAGCATGTGCaa                                                >  1:1160896/1‑67 (MQ=255)                            cccTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                         >  1:2677275/1‑71 (MQ=255)                            cccTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                         <  1:1124034/71‑1 (MQ=255)                            cccTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                         <  1:3800265/71‑1 (MQ=255)                            cccTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                         <  1:2542447/71‑1 (MQ=255)                            cccTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                         <  1:3535310/71‑1 (MQ=255)                            cccTGAAGACTGATGACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                         <  1:249573/71‑1 (MQ=255)                              cTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTc                                            >  1:1675362/1‑66 (MQ=255)                                gatgaCTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCa                                                       >  1:1821765/1‑53 (MQ=255)                                 atgaCTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTc                                            >  1:299008/1‑63 (MQ=255)                                 atgaCTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGa                                       >  1:3544750/1‑68 (MQ=255)                                 atgaCTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTa                                    >  1:2037497/1‑71 (MQ=255)                                  tgaCTGATCACCCACGCCAACAATACTTGTGCCGCTCTGATATTGTGAGCATGTGCAATTTCGTTGACTAc                                   >  1:2786697/1‑71 (MQ=255)                                      tGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                         <  1:1801200/61‑1 (MQ=255)                                        aTCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCg                                           >  1:77488/1‑57 (MQ=255)                                        aTCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtt                             <  1:2869771/71‑1 (MQ=255)                                         tCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGttt                            >  1:3279582/1‑71 (MQ=255)                                         tCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtt                             <  1:1773975/70‑1 (MQ=255)                                            cccACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttttt                         <  1:3881607/71‑1 (MQ=255)                                            cccACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttttt                         <  1:3056087/71‑1 (MQ=255)                                            cccACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGtttttt                         <  1:4205292/71‑1 (MQ=255)                                            cccACGCCAACAATACTTGTGCAGCGCTGATATTGTGAGCATGTGCAAttt                                             <  1:1246170/51‑1 (MQ=255)                                             ccACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGtt                                         >  1:2615409/1‑54 (MQ=255)                                             ccACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTa                        <  1:3624159/71‑1 (MQ=255)                                              cACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCg                                           <  1:4409560/51‑1 (MQ=255)                                              cACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTaa                       >  1:1351709/1‑71 (MQ=255)                                              cACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTaa                       >  1:1337675/1‑71 (MQ=255)                                                   caacaaTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTc                  <  1:654460/71‑1 (MQ=255)                                                     acaaTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTc                  <  1:2949006/69‑1 (MQ=255)                                                     acaaTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTc                  <  1:2606363/69‑1 (MQ=255)                                                     acaaTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTc                  <  1:1190901/69‑1 (MQ=255)                                                      caaTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACcg                                 >  1:2219248/1‑53 (MQ=255)                                                       aaTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCAtt              >  1:1088523/1‑71 (MQ=255)                                                       aaTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCAtt              >  1:4053718/1‑71 (MQ=255)                                                        aTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGt                                          >  1:2127692/1‑42 (MQ=255)                                                        aTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGa                                       >  1:1604515/1‑45 (MQ=255)                                                        aTACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGa                                       >  1:1346606/1‑45 (MQ=255)                                                         tACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAAc                      >  1:1147756/1‑61 (MQ=255)                                                          aCTTGTGCCGAACTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTaa                       <  1:915122/59‑1 (MQ=255)                                                           cTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTgcg           >  1:4142158/1‑70 (MQ=255)                                                           cTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTAGTTGACTACCGCGTTTTTTAACAGTCCATTgcg           >  1:3773779/1‑70 (MQ=255)                                                              gtgCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCa         <  1:3589912/69‑1 (MQ=255)                                                                 ccGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGc    >  1:1094333/1‑71 (MQ=255)                                                                   gcgcTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGccc  <  1:3372102/71‑1 (MQ=255)                                                                     gcTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCaa        >  1:1911491/1‑63 (MQ=255)                                                                     |                                                                     GTGGCCGCTTTTGGAATCGCCATCACACCCTGATGACTGATCACCCACGCCAACAATACTTGTGCCGCGCTGATATTGTGAGCATGTGCAATTTCGTTGACTACCGCGTTTTTTAACAGTCCATTGCGCAACCGCCC  >  W3110S.gb/1866617‑1866753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑