| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,969,214 | G→A | 8.2% | S334S (AGC→AGT) | cheB ← | fused chemotaxis regulator and protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,969,214 | 0 | G | A | 8.2% | 217.8 / 7.0 | 73 | S334S (AGC→AGT) | cheB | fused chemotaxis regulator and protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Reads supporting (aligned to +/- strand): ref base G (40/27); new base A (3/3); total (43/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
TCCGCCATTTCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACA > W3110S.gb/1969144‑1969284 | tCCGCCATTTCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTgg < 1:1241817/71‑1 (MQ=255)tCCGCCATTTCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTgg < 1:1347037/71‑1 (MQ=255) cGCCATTTCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCt < 1:1203221/71‑1 (MQ=255) cGCCATTTCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCt > 1:2828167/1‑71 (MQ=255) gCCATTTCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCt > 1:3810316/1‑70 (MQ=255) tttCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTAc < 1:3410372/69‑1 (MQ=255) tttCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCt < 1:354546/71‑1 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTgg > 1:3349455/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTgg > 1:3363604/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTgg > 1:2260081/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTgg > 1:4116927/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTgg > 1:1989009/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTgg > 1:1252787/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTgg > 1:1260862/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTgg > 1:584193/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTgg > 1:1391771/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTAACTgg > 1:1917115/1‑71 (MQ=255) tCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCAGGCTTACCTgg > 1:1387932/1‑71 (MQ=255) acacTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGc > 1:3621944/1‑70 (MQ=255) acacTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCt < 1:904098/71‑1 (MQ=255) cacTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTa < 1:1547633/71‑1 (MQ=255) cTCCTGATTTAAATACGTATCGCCTGTGCGGCACTAATTTTTGCCAACATTTGCTGGCTTAGCTGGCTAAg < 1:1767871/71‑1 (MQ=255) cTCCTGATTTAAATACGTATCGCCTGTGCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAg < 1:1762721/71‑1 (MQ=255) cTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAg < 1:607852/71‑1 (MQ=255) cTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAg < 1:2217153/71‑1 (MQ=255) cTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAg < 1:858955/71‑1 (MQ=255) cTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGc > 1:2013751/1‑55 (MQ=255) cTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATc < 1:2652478/71‑1 (MQ=255) tGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATc > 1:731523/1‑70 (MQ=255) aTTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGa > 1:70374/1‑70 (MQ=255) aTTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGAc > 1:748677/1‑71 (MQ=255) aTTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGAc > 1:1404444/1‑71 (MQ=255) aTTTAAATACGTATCGCCTGTCCGACACTAATTTTTGCCAACATTTGCTGGCtt < 1:1757511/54‑1 (MQ=255) ttAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCa > 1:3264436/1‑71 (MQ=255) tAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCa > 1:2395862/1‑70 (MQ=255) tAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCa > 1:301379/1‑70 (MQ=255) cGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCa < 1:1977846/71‑1 (MQ=255) cGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCa < 1:1013242/71‑1 (MQ=255) gTATCGCCTGGCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAg < 1:427468/71‑1 (MQ=255) gCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGacac < 1:3159538/70‑1 (MQ=255) cTGTCCGTCACTTATTTTTGGCAACATTTGCTGGCTTACCTGGCTa < 1:223025/46‑1 (MQ=37) gCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATAtt < 1:193252/71‑1 (MQ=255) gCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATAtt < 1:1508648/71‑1 (MQ=255) cACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTg < 1:2864361/71‑1 (MQ=255) cTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACt < 1:2326854/48‑1 (MQ=255) tAATTTTTGCCTACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAg < 1:3168008/53‑1 (MQ=255) tAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCa < 1:3545539/52‑1 (MQ=255) tAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATg > 1:4037742/1‑71 (MQ=255) aaTTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGa > 1:4256384/1‑53 (MQ=255) aaTTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGa > 1:1820258/1‑53 (MQ=255) aTTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGc > 1:974217/1‑71 (MQ=255) aTTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGc > 1:3860181/1‑71 (MQ=255) tttttGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGcc < 1:1790508/71‑1 (MQ=255) ttttGCCAACATTTGCTGGCTTACCTGGCTAAGATCGa > 1:2400176/1‑38 (MQ=255) tttGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCt > 1:3554429/1‑70 (MQ=255) tGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGAt > 1:4020352/1‑63 (MQ=255) ccAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGTCACCACCCATAtt < 1:3230374/58‑1 (MQ=255) cagcATTTGCTGACTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTcg < 1:2274981/64‑1 (MQ=255) cagcATTTGCTGACTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTcg < 1:2024765/64‑1 (MQ=255) cagcATTTGCTGACTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTcg < 1:1017841/64‑1 (MQ=255) aaCATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCa < 1:963314/41‑1 (MQ=255) aaCATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCgg > 1:2704712/1‑71 (MQ=255) aaCATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCgg > 1:2888089/1‑71 (MQ=255) aaCATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCgg > 1:1472111/1‑71 (MQ=255) aaCATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCgg < 1:1409376/71‑1 (MQ=255) gcATTTGCTGACTTACCTGGCTAAGATCGACCACTTCGCAGAcacc > 1:1757376/2‑46 (MQ=255) gcATTTGCTGACTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGa > 1:2816792/2‑57 (MQ=255) gcATTTGCTGACTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGa > 1:2611987/2‑57 (MQ=255) aTTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCAt > 1:3877915/1‑71 (MQ=255) aTTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCAt > 1:249660/1‑71 (MQ=255) aTTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCAt > 1:190485/1‑71 (MQ=255) ttGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGa > 1:9968/1‑53 (MQ=255) ttGCTGGCTTACCTGGCTAAGATCGACCACTTAGCAGCCACCACCCATATTGATGGCCTCGCGCGGCATGc > 1:4010979/1‑71 (MQ=255) tGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCa > 1:2715238/1‑46 (MQ=255) tGCTGGCTTACCTGGCTAAGATCCACCACTTCGCa > 1:4207017/1‑35 (MQ=255) gCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAaca > 1:1856044/1‑71 (MQ=255) | TCCGCCATTTCACACTCCTGATTTAAATACGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACA > W3110S.gb/1969144‑1969284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |