Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 2,079,831 | T→G | 5.3% | P42P (CCT→CCG) | yeeV → | toxin of the YeeV‑YeeU toxin‑antitoxin system |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,079,831 | 0 | T | G | 5.3% | 239.1 / 7.9 | 95 | P42P (CCT→CCG) | yeeV | toxin of the YeeV‑YeeU toxin‑antitoxin system |
Reads supporting (aligned to +/- strand): ref base T (46/44); new base G (2/3); total (48/47) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 |
Only 100 of 101 total aligned reads displayed.
GAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTT > W3110S.gb/2079763‑2079900 | gAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCttt > 1:2928246/1‑71 (MQ=255) aaaTCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCtttt < 1:2556607/71‑1 (MQ=255) aaTCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTg < 1:3255204/71‑1 (MQ=255) tCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGcc > 1:432551/1‑71 (MQ=255) tGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGa > 1:756315/1‑71 (MQ=255) tGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGa > 1:685188/1‑71 (MQ=255) tGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGa > 1:4173033/1‑71 (MQ=255) gCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCttt < 1:1323323/63‑1 (MQ=255) aGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa < 1:1649121/70‑1 (MQ=255) aTACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACg < 1:1252463/71‑1 (MQ=255) aTACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACg > 1:3162973/1‑71 (MQ=255) aTACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACg < 1:4056695/71‑1 (MQ=255) tACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGc > 1:139279/1‑61 (MQ=255) tACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgt > 1:2477032/1‑71 (MQ=255) aCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGc > 1:1425717/1‑60 (MQ=255) aCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGc > 1:1784706/1‑60 (MQ=255) aCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgtg > 1:1794628/1‑71 (MQ=255) aCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgt < 1:3162082/70‑1 (MQ=255) ctgctgTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgtgt < 1:2703809/71‑1 (MQ=255) tgctgTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGcc < 1:649994/59‑1 (MQ=255) ctgTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAt < 1:2407423/71‑1 (MQ=255) tCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt < 1:4396016/69‑1 (MQ=255) tCCCGACTGCTGGACAAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt < 1:1458029/69‑1 (MQ=255) ccGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag > 1:2040078/1‑70 (MQ=255) ccGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag > 1:95700/1‑70 (MQ=255) cGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagca < 1:3165006/71‑1 (MQ=255) gACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcag > 1:3649580/1‑71 (MQ=255) ctgctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGTTTGAgcagca < 1:1206695/71‑1 (MQ=255) ctgctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAgcagca < 1:1819737/71‑1 (MQ=255) ctgctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAgcagca < 1:3979646/71‑1 (MQ=255) ctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCatat > 1:2990244/1‑71 (MQ=255) ctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCat < 1:2994881/69‑1 (MQ=255) ctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCat < 1:2285527/69‑1 (MQ=255) tgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcag > 1:3919890/1‑65 (MQ=255) gACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATTAACGTGTGATTGAGCAGCATATTg < 1:1387441/70‑1 (MQ=255) cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa < 1:3109275/44‑1 (MQ=255) cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa < 1:265462/44‑1 (MQ=255) cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa < 1:4006801/44‑1 (MQ=255) cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGa > 1:3880559/1‑68 (MQ=255) cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGa > 1:4116576/1‑68 (MQ=255) cTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgtgt < 1:2407649/45‑1 (MQ=255) cTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGaggcaggc < 1:763449/70‑1 (MQ=255) cTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGaggcaggc < 1:3984694/70‑1 (MQ=255) tATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAt > 1:257074/1‑71 (MQ=255) aTGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAtt > 1:2171244/1‑71 (MQ=255) tGGCCTCACACTGAATGACACACCTTTTGCCGATGAAc > 1:2958447/1‑38 (MQ=255) tGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCata > 1:3756379/1‑56 (MQ=255) tGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAttt < 1:3057903/71‑1 (MQ=255) cggCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAttt > 1:4047498/2‑71 (MQ=255) cggCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAttt > 1:586309/2‑71 (MQ=255) ggCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc < 1:846866/71‑1 (MQ=255) ggCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc < 1:3794467/71‑1 (MQ=255) ggCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc < 1:841756/71‑1 (MQ=255) gCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc < 1:1221433/70‑1 (MQ=255) gCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc > 1:870502/1‑70 (MQ=255) gCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa < 1:933425/71‑1 (MQ=255) tCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcagc > 1:1272980/1‑48 (MQ=255) tCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagca < 1:2600946/46‑1 (MQ=255) tCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc < 1:1679667/67‑1 (MQ=255) tCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc < 1:3453590/67‑1 (MQ=255) cacaCTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgt > 1:4266470/1‑71 (MQ=255) acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcagc > 1:3188518/1‑46 (MQ=255) acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcagc > 1:504159/1‑46 (MQ=255) acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAgcagca < 1:579038/47‑1 (MQ=255) acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGag > 1:3555918/1‑54 (MQ=255) acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa > 1:2613341/1‑66 (MQ=255) acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa > 1:2738800/1‑66 (MQ=255) acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtg > 1:1309985/1‑71 (MQ=255) acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtg > 1:3471797/1‑71 (MQ=255) cacTGAATGACACACCTTTTGCCGATGAACGTGTGAt > 1:4088204/1‑37 (MQ=255) cacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtgt < 1:379819/71‑1 (MQ=255) cacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtgt > 1:51211/1‑71 (MQ=255) cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag > 1:1428243/1‑39 (MQ=255) cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCatat < 1:2028262/47‑1 (MQ=255) cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGaggca > 1:1441148/1‑54 (MQ=255) cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgt < 1:300389/67‑1 (MQ=255) cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGa < 1:910721/71‑1 (MQ=255) cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGa > 1:445695/1‑71 (MQ=255) tGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATAtt < 1:1106047/47‑1 (MQ=255) tGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGAt > 1:3391834/1‑71 (MQ=255) gAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgt < 1:1535525/65‑1 (MQ=255) gAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgt < 1:2169631/65‑1 (MQ=255) aaTGACACACCTTTTGCCGATGAACGTGTGATTGagcagc < 1:3723392/40‑1 (MQ=255) aaTGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAggcaggca > 1:266283/1‑55 (MQ=255) aaTGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAggcaggca > 1:2137510/1‑55 (MQ=255) aaTGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGc > 1:726222/1‑71 (MQ=255) acacacCTTTTGCCGATGAACGTGTGATTGAgcagca < 1:1114063/37‑1 (MQ=255) acacacCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGt > 1:1551588/1‑70 (MQ=255) cacacCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGa < 1:2354068/71‑1 (MQ=255) acacCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGaa > 1:1582171/1‑71 (MQ=255) acCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACt > 1:4131532/1‑71 (MQ=255) acCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACt > 1:905477/1‑71 (MQ=255) acCTTTTGCCGATGAACCTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACt < 1:2260539/71‑1 (MQ=255) ccTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGagg < 1:3267602/40‑1 (MQ=255) ccTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGa > 1:229254/1‑38 (MQ=255) ccTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCCGTGAACtt < 1:2651655/71‑1 (MQ=255) cTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACttt < 1:2384160/71‑1 (MQ=255) ttttGCCGATGAACGTGTGATTGAGCAGCATATTGaggca < 1:1510686/40‑1 (MQ=255) ttttGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc < 1:1675980/48‑1 (MQ=255) ttttGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACt < 1:1955359/51‑1 (MQ=255) | GAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTT > W3110S.gb/2079763‑2079900 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |