Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,079,831 T→G 5.3% P42P (CCT→CCG yeeV → toxin of the YeeV‑YeeU toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,079,8310TG 5.3% 239.1 / 7.9 95P42P (CCT→CCGyeeVtoxin of the YeeV‑YeeU toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (46/44);  new base G (2/3);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 101 total aligned reads displayed.

GAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTT  >  W3110S.gb/2079763‑2079900
                                                                    |                                                                     
gAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCttt                                                                     >  1:2928246/1‑71 (MQ=255)
 aaaTCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCtttt                                                                    <  1:2556607/71‑1 (MQ=255)
  aaTCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTg                                                                   <  1:3255204/71‑1 (MQ=255)
    tCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGcc                                                                 >  1:432551/1‑71 (MQ=255)
      tGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGa                                                               >  1:756315/1‑71 (MQ=255)
      tGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGa                                                               >  1:685188/1‑71 (MQ=255)
      tGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGa                                                               >  1:4173033/1‑71 (MQ=255)
        gCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCttt                                                                     <  1:1323323/63‑1 (MQ=255)
          aGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa                                                            <  1:1649121/70‑1 (MQ=255)
            aTACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACg                                                         <  1:1252463/71‑1 (MQ=255)
            aTACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACg                                                         >  1:3162973/1‑71 (MQ=255)
            aTACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACg                                                         <  1:4056695/71‑1 (MQ=255)
             tACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGc                                                                  >  1:139279/1‑61 (MQ=255)
             tACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgt                                                        >  1:2477032/1‑71 (MQ=255)
              aCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGc                                                                  >  1:1425717/1‑60 (MQ=255)
              aCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGc                                                                  >  1:1784706/1‑60 (MQ=255)
              aCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgtg                                                       >  1:1794628/1‑71 (MQ=255)
              aCTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgt                                                        <  1:3162082/70‑1 (MQ=255)
               ctgctgTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgtgt                                                      <  1:2703809/71‑1 (MQ=255)
                tgctgTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGcc                                                                 <  1:649994/59‑1 (MQ=255)
                  ctgTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAt                                                   <  1:2407423/71‑1 (MQ=255)
                     tCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt                                                  <  1:4396016/69‑1 (MQ=255)
                     tCCCGACTGCTGGACAAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt                                                  <  1:1458029/69‑1 (MQ=255)
                       ccGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag                                               >  1:2040078/1‑70 (MQ=255)
                       ccGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag                                               >  1:95700/1‑70 (MQ=255)
                        cGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagca                                             <  1:3165006/71‑1 (MQ=255)
                         gACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcag                                            >  1:3649580/1‑71 (MQ=255)
                           ctgctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGTTTGAgcagca                                          <  1:1206695/71‑1 (MQ=255)
                           ctgctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAgcagca                                          <  1:1819737/71‑1 (MQ=255)
                           ctgctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAgcagca                                          <  1:3979646/71‑1 (MQ=255)
                              ctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCatat                                       >  1:2990244/1‑71 (MQ=255)
                              ctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCat                                         <  1:2994881/69‑1 (MQ=255)
                              ctgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCat                                         <  1:2285527/69‑1 (MQ=255)
                               tgGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcag                                            >  1:3919890/1‑65 (MQ=255)
                                 gACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATTAACGTGTGATTGAGCAGCATATTg                                     <  1:1387441/70‑1 (MQ=255)
                                    cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa                                                            <  1:3109275/44‑1 (MQ=255)
                                    cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa                                                            <  1:265462/44‑1 (MQ=255)
                                    cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGa                                                            <  1:4006801/44‑1 (MQ=255)
                                    cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGa                                    >  1:3880559/1‑68 (MQ=255)
                                    cAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGa                                    >  1:4116576/1‑68 (MQ=255)
                                         cTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACgtgt                                                      <  1:2407649/45‑1 (MQ=255)
                                         cTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGaggcaggc                             <  1:763449/70‑1 (MQ=255)
                                         cTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGaggcaggc                             <  1:3984694/70‑1 (MQ=255)
                                          tATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAt                           >  1:257074/1‑71 (MQ=255)
                                           aTGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAtt                          >  1:2171244/1‑71 (MQ=255)
                                            tGGCCTCACACTGAATGACACACCTTTTGCCGATGAAc                                                          >  1:2958447/1‑38 (MQ=255)
                                            tGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCata                                        >  1:3756379/1‑56 (MQ=255)
                                            tGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAttt                         <  1:3057903/71‑1 (MQ=255)
                                            cggCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAttt                         >  1:4047498/2‑71 (MQ=255)
                                            cggCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCAttt                         >  1:586309/2‑71 (MQ=255)
                                             ggCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc                        <  1:846866/71‑1 (MQ=255)
                                             ggCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc                        <  1:3794467/71‑1 (MQ=255)
                                             ggCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc                        <  1:841756/71‑1 (MQ=255)
                                              gCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc                        <  1:1221433/70‑1 (MQ=255)
                                              gCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc                        >  1:870502/1‑70 (MQ=255)
                                              gCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa                       <  1:933425/71‑1 (MQ=255)
                                                 tCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcagc                                           >  1:1272980/1‑48 (MQ=255)
                                                 tCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagca                                             <  1:2600946/46‑1 (MQ=255)
                                                 tCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc                        <  1:1679667/67‑1 (MQ=255)
                                                 tCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc                        <  1:3453590/67‑1 (MQ=255)
                                                  cacaCTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgt                   >  1:4266470/1‑71 (MQ=255)
                                                   acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcagc                                           >  1:3188518/1‑46 (MQ=255)
                                                   acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcagc                                           >  1:504159/1‑46 (MQ=255)
                                                   acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAgcagca                                          <  1:579038/47‑1 (MQ=255)
                                                   acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGag                                   >  1:3555918/1‑54 (MQ=255)
                                                   acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa                       >  1:2613341/1‑66 (MQ=255)
                                                   acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa                       >  1:2738800/1‑66 (MQ=255)
                                                   acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtg                  >  1:1309985/1‑71 (MQ=255)
                                                   acacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtg                  >  1:3471797/1‑71 (MQ=255)
                                                    cacTGAATGACACACCTTTTGCCGATGAACGTGTGAt                                                   >  1:4088204/1‑37 (MQ=255)
                                                    cacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtgt                 <  1:379819/71‑1 (MQ=255)
                                                    cacTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgtgt                 >  1:51211/1‑71 (MQ=255)
                                                      cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag                                               >  1:1428243/1‑39 (MQ=255)
                                                      cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCatat                                       <  1:2028262/47‑1 (MQ=255)
                                                      cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGaggca                                >  1:1441148/1‑54 (MQ=255)
                                                      cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgt                   <  1:300389/67‑1 (MQ=255)
                                                      cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGa               <  1:910721/71‑1 (MQ=255)
                                                      cTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGa               >  1:445695/1‑71 (MQ=255)
                                                       tGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATAtt                                      <  1:1106047/47‑1 (MQ=255)
                                                       tGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGAt              >  1:3391834/1‑71 (MQ=255)
                                                        gAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgt                   <  1:1535525/65‑1 (MQ=255)
                                                        gAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACtgt                   <  1:2169631/65‑1 (MQ=255)
                                                         aaTGACACACCTTTTGCCGATGAACGTGTGATTGagcagc                                           <  1:3723392/40‑1 (MQ=255)
                                                         aaTGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAggcaggca                            >  1:266283/1‑55 (MQ=255)
                                                         aaTGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAggcaggca                            >  1:2137510/1‑55 (MQ=255)
                                                         aaTGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGc            >  1:726222/1‑71 (MQ=255)
                                                             acacacCTTTTGCCGATGAACGTGTGATTGAgcagca                                          <  1:1114063/37‑1 (MQ=255)
                                                             acacacCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGt         >  1:1551588/1‑70 (MQ=255)
                                                              cacacCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGa       <  1:2354068/71‑1 (MQ=255)
                                                               acacCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGaa      >  1:1582171/1‑71 (MQ=255)
                                                                 acCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACt    >  1:4131532/1‑71 (MQ=255)
                                                                 acCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACt    >  1:905477/1‑71 (MQ=255)
                                                                 acCTTTTGCCGATGAACCTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACt    <  1:2260539/71‑1 (MQ=255)
                                                                  ccTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGagg                                  <  1:3267602/40‑1 (MQ=255)
                                                                  ccTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGa                                    >  1:229254/1‑38 (MQ=255)
                                                                  ccTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCCGTGAACtt   <  1:2651655/71‑1 (MQ=255)
                                                                   cTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACttt  <  1:2384160/71‑1 (MQ=255)
                                                                    ttttGCCGATGAACGTGTGATTGAGCAGCATATTGaggca                                <  1:1510686/40‑1 (MQ=255)
                                                                    ttttGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTc                        <  1:1675980/48‑1 (MQ=255)
                                                                    ttttGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACt                     <  1:1955359/51‑1 (MQ=255)
                                                                    |                                                                     
GAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTT  >  W3110S.gb/2079763‑2079900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: