| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,341,925 | G→A | 8.9% | R722R (CGC→CGT) | gyrA ← | DNA gyrase (type II topoisomerase), subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,341,925 | 0 | G | A | 8.9% | 165.2 / 5.0 | 56 | R722R (CGC→CGT) | gyrA | DNA gyrase (type II topoisomerase), subunit A |
| Reads supporting (aligned to +/- strand): ref base G (31/20); new base A (2/3); total (33/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
AGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCT > W3110S.gb/2341857‑2341991 | aGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACcgcg < 1:21000/71‑1 (MQ=255) ggATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACcgcg < 1:1308687/70‑1 (MQ=255) ttGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCGCCTAAACGAATACCACGAaca > 1:4283143/1‑71 (MQ=255) ttGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCGCCTAAACGAATACCACGAaca > 1:4277951/1‑71 (MQ=255) cgcCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACAcc < 1:1543110/70‑1 (MQ=255) ccATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCggt < 1:3118046/71‑1 (MQ=255) cATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCggtg > 1:2531845/1‑71 (MQ=255) tCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCggtggt > 1:979756/1‑71 (MQ=255) gCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGgtgt < 1:2236340/71‑1 (MQ=255) aCGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTg > 1:3211023/1‑70 (MQ=255) aGGCACGATCAGAGAGACGACTTTATCGCCTTAACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGc > 1:1941865/1‑71 (MQ=255) ggCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCggtggt > 1:3037985/1‑62 (MQ=255) tcACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCa < 1:3822439/66‑1 (MQ=255) gCACGATCAGAGAGACGACTTTATCGCCTTCGCCTAAACGAATACCACGAACACCGGTGGTGTTGCAGccc < 1:3668633/71‑1 (MQ=255) gCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCggt < 1:3498472/58‑1 (MQ=255) gCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCggt < 1:3493553/58‑1 (MQ=255) aCGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCggtg > 1:4045489/1‑57 (MQ=255) gATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCg < 1:2969344/71‑1 (MQ=255) aTCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGc < 1:4418235/71‑1 (MQ=255) cAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCAc > 1:248444/1‑71 (MQ=255) gagaCGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGa > 1:1239629/1‑70 (MQ=255) gagaCGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGAc < 1:2127340/71‑1 (MQ=255) agaCGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGgtgt < 1:2973484/51‑1 (MQ=255) agaCGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCg < 1:3084757/63‑1 (MQ=255) cgacTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGc < 1:733138/61‑1 (MQ=255) cgacTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACagaa < 1:2234132/71‑1 (MQ=255) tATCGCCTTCGCCTAAACGAATACCACGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGAct < 1:3721397/69‑1 (MQ=255) tATCGCCTTCGCCTAAACGAATACCACGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGAct < 1:3092603/69‑1 (MQ=255) tATCGCCTTCACCTAAGCGAATACCGCGAACACCggt > 1:401298/1‑37 (MQ=255) tATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCa > 1:2677080/1‑46 (MQ=255) tATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCa > 1:616134/1‑46 (MQ=255) tATCGCCTTCACCTAAGCGAATACAGCGAACACCGGTGGTGTTGc > 1:1933178/1‑45 (MQ=255) tCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCtt > 1:3396422/1‑70 (MQ=255) gCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTaa < 1:1289572/71‑1 (MQ=255) gCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTa < 1:4246012/70‑1 (MQ=255) ccTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGcac > 1:3818918/1‑71 (MQ=255) tAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCa > 1:639064/1‑43 (MQ=255) tAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCAc > 1:3483744/1‑44 (MQ=255) tAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACagaaga > 1:2816574/1‑54 (MQ=255) tAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAg > 1:558506/1‑64 (MQ=255) tAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGcacc > 1:17233/1‑70 (MQ=255) aaGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGcaccac > 1:2414796/1‑71 (MQ=255) aaGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGcaccac > 1:2462954/1‑71 (MQ=255) aaGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGcaccac > 1:1262262/1‑71 (MQ=255) aaGCGAATACCGAGAACACCGGTGGTGTTGCAGCCCATCGCACGGACagaaga > 1:814238/1‑53 (MQ=255) gCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACtt < 1:3078075/71‑1 (MQ=255) cGAATACCGCGAACACCGGTGGTGTTTCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACttt < 1:1622220/71‑1 (MQ=255) cGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACagaaga > 1:3111937/1‑50 (MQ=255) cGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACttt < 1:2492346/71‑1 (MQ=255) cGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACtt > 1:355252/1‑70 (MQ=255) gAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACttt < 1:922424/70‑1 (MQ=255) gAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTa > 1:2294938/1‑71 (MQ=255) aTACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTAcc > 1:363930/1‑71 (MQ=255) tACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACttt < 1:612275/67‑1 (MQ=255) tACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTAc > 1:2818241/1‑69 (MQ=255) tACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTAc > 1:1655077/1‑69 (MQ=255) ccGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGcacca > 1:995165/1‑61 (MQ=255) ccGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCt > 1:632297/1‑69 (MQ=255) | AGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGAAGACTCTTTAAAGCGCACCACTTTACCT > W3110S.gb/2341857‑2341991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |