| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,643,819 | C→T | 12.8% | G721G (GGG→GGA) | pbpC ← | fused transglycosylase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,643,819 | 0 | C | T | 12.8% | 100.5 / 5.9 | 39 | G721G (GGG→GGA) | pbpC | fused transglycosylase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (15/19); new base T (3/2); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 | |||||||||||
TCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGC > W3110S.gb/2643758‑2643889 | tCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACtt < 1:314595/71‑1 (MQ=255) cgcgTTCAGTTAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACtt < 1:2270161/59‑1 (MQ=255) cgcgTTCAGTTAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGc < 1:338432/71‑1 (MQ=255) gcgTTCAGTTAACGGTTCGCGATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCa < 1:1105888/71‑1 (MQ=255) gcgTTCAGTTAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGc > 1:4018977/1‑70 (MQ=255) gcgTTCAGTTAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCa < 1:678311/71‑1 (MQ=255) ttCAGTTAACGGTTCGCCGTTCAGAAACCACCAGCGTTCACCTGCTCCTCCACTTGa < 1:2913066/57‑1 (MQ=255) ttCAGTTAACGGTTCGCCGTTCAGAAACCACCAGCGTTCACCTGCTCCTCCACTTGa < 1:4410419/57‑1 (MQ=255) ttCAGTTAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAg < 1:2068613/71‑1 (MQ=255) ttCAGTTAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAg < 1:2543242/71‑1 (MQ=255) ttAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCt < 1:4198281/71‑1 (MQ=255) tAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCaa > 1:1744620/1‑57 (MQ=255) aaCGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGa > 1:3254629/1‑50 (MQ=255) aaCGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGa > 1:4071139/1‑50 (MQ=255) aaCGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTc > 1:3140789/1‑71 (MQ=255) aCGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTcc < 1:775865/71‑1 (MQ=255) aCGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTc < 1:2972807/70‑1 (MQ=255) gTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCt < 1:1392754/71‑1 (MQ=255) gCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTg < 1:3682498/41‑1 (MQ=255) cATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGccc > 1:3589316/1‑71 (MQ=255) ttCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCgg > 1:1660159/1‑71 (MQ=255) tCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCa < 1:1479226/48‑1 (MQ=255) tCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGc > 1:109899/1‑61 (MQ=255) cAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGt > 1:3031961/1‑70 (MQ=255) cAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGt > 1:3994913/1‑70 (MQ=255) cAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGt > 1:3082431/1‑70 (MQ=255) cAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCAGCGCCCGGt > 1:3518856/1‑70 (MQ=255) aGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTa < 1:3466671/70‑1 (MQ=255) ccaccaGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGtt < 1:3793817/71‑1 (MQ=255) accaGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTta < 1:4154499/71‑1 (MQ=255) caGCGTTCACCTGCTCCTCCACTTGATTGCAACGGCaaa > 1:223982/1‑39 (MQ=255) caGCGTTCACCTGCTCCTCCACTTGATTGCAACGGCaaa > 1:1536388/1‑39 (MQ=255) caGCGTTCACCTGCTCCTCCACTTGATTGCAACGGCaaa > 1:1056105/1‑39 (MQ=255) cGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTtaataa < 1:2097189/70‑1 (MQ=255) gTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATc < 1:361605/71‑1 (MQ=255) tCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGc > 1:2277382/1‑38 (MQ=255) ccTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCgcgc > 1:1696422/1‑70 (MQ=255) cTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAAc < 1:213349/54‑1 (MQ=255) gCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGcc > 1:4172666/1‑68 (MQ=255) cccTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTTAACGTTTAATAATCGCGCCATcgc < 1:2649228/71‑1 (MQ=255) cccTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATcgc > 1:2092694/1‑71 (MQ=255) | TCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGC > W3110S.gb/2643758‑2643889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |