| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,696,316 | A→G | 12.0% | G77G (GGT→GGC) | tadA ← | tRNA‑specific adenosine deaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,696,316 | 0 | A | G | 12.0% | 119.9 / 8.9 | 50 | G77G (GGT→GGC) | tadA | tRNA‑specific adenosine deaminase |
| Reads supporting (aligned to +/- strand): ref base A (16/28); new base G (4/2); total (20/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01 | |||||||||||
ACATTACACATGGTTCAAGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGACCAATCGGGCGGTTCCAGCC > W3110S.gb/2696246‑2696384 | acaTTACACATGGTTCAAGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGacca < 1:2370468/71‑1 (MQ=255) acacATGGTTCAAGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTg > 1:1705403/1‑71 (MQ=255) acacATGGTTCAAGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTg > 1:1706906/1‑71 (MQ=255) acaTGGTTCAAGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTccc > 1:4056560/1‑71 (MQ=255) aTGGTTCAAGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGc < 1:3622797/71‑1 (MQ=255) tGGTTCAAGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCg < 1:47057/69‑1 (MQ=255) cAAGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAggg > 1:3985758/1‑69 (MQ=255) aaGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGcc < 1:3143841/70‑1 (MQ=255) aGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCAt < 1:1783590/71‑1 (MQ=255) aGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCAt < 1:2113897/71‑1 (MQ=255) gTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGAt < 1:4409675/71‑1 (MQ=255) gTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGAt < 1:1887225/71‑1 (MQ=255) aCATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGAttt < 1:2824627/70‑1 (MQ=255) tACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGaccac < 1:3939353/46‑1 (MQ=255) tACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCAt < 1:3010541/62‑1 (MQ=255) aCAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCGCCCTGCCGTAggg > 1:3297314/1‑57 (MQ=255) aCAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCGCCCTGCCGTAggg > 1:4295890/1‑57 (MQ=255) aCAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCGCCCTGCCGTAggg > 1:1402392/1‑57 (MQ=255) aCAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCGCCCTGCCGTAggg > 1:3661631/1‑57 (MQ=255) cAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGacca > 1:2355238/1‑43 (MQ=255) cAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTg > 1:965/1‑68 (MQ=255) cAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCa > 1:2020975/1‑70 (MQ=255) cAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCa > 1:2818913/1‑70 (MQ=255) cAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCAt > 1:1410841/1‑71 (MQ=255) cAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCAt > 1:3225622/1‑71 (MQ=255) tGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATg < 1:2601543/56‑1 (MQ=255) tGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCg < 1:159153/71‑1 (MQ=255) gCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGt < 1:1637944/71‑1 (MQ=255) tCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTg < 1:924854/58‑1 (MQ=255) gATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGAt < 1:2370685/71‑1 (MQ=255) gATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGAt < 1:2687500/71‑1 (MQ=255) gATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGAt < 1:1026095/71‑1 (MQ=255) gATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGACCATGATTTCTGCAt > 1:1836395/1‑59 (MQ=255) tCAGACGATAATTTTGCATCACCAGACCACCCTGcc < 1:2816347/36‑1 (MQ=255) cAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGAt < 1:471081/48‑1 (MQ=255) cAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCAt > 1:3321545/1‑56 (MQ=255) aGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCAt < 1:3162480/70‑1 (MQ=255) aGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCAt < 1:1795135/70‑1 (MQ=255) aCGATAATTTTGCATCACCAGAACACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGc < 1:1185858/71‑1 (MQ=255) cGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCg < 1:809719/71‑1 (MQ=255) gATAATTTTGCATCACGAGACCACCGTGCCGCAGGGCCATGATTTCTGCATGTGCGGt < 1:3627464/58‑1 (MQ=39) gATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCg < 1:477797/56‑1 (MQ=255) tAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGa > 1:347736/1‑40 (MQ=255) tAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCAtgtg > 1:1376759/1‑52 (MQ=255) aaTTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGAt > 1:192250/1‑40 (MQ=255) ttttGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGACCAAt > 1:1978768/1‑71 (MQ=255) ttttGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGACCAAt < 1:1187049/71‑1 (MQ=255) ttttGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGT‑GGATCATGGCGACCAATc < 1:2198455/71‑1 (MQ=255) gCATCTCCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGAt < 1:3846857/54‑1 (MQ=255) aTCACCAGACCGCCCTGCCGTAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGTCGACCAATCGGACg < 1:3948464/71‑1 (MQ=255) tCACCAGACCGCCCTGCCGTAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGTCGACCAATCGGACg < 1:2429601/70‑1 (MQ=255) cACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGACCAATCGGGCGGt > 1:402648/1‑71 (MQ=255) aCCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGACCAATCGGGCGGt < 1:4042151/70‑1 (MQ=255) accaccCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGACCAATCGGGCGGTTCCAGc > 1:1881376/1‑71 (MQ=255) ccaccCTTCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGACCAATCGGGCGGTTCCAGcc < 1:2788670/71‑1 (MQ=255) ccaccCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGACCAATCGGGCGGTTACAGcc < 1:1939323/71‑1 (MQ=255) caccCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGAcc < 1:3731863/52‑1 (MQ=255) | ACATTACACATGGTTCAAGCGTGACATACAACGTGGCGTCGATCAGACGATAATTTTGCATCACCAGACCACCCTGCCGCAGGGCCATGATTTCTGCATGTGCGGTGGGATCATGGCGACCAATCGGGCGGTTCCAGCC > W3110S.gb/2696246‑2696384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |