| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,747,558 | A→G | 6.2% | K13K (AAA→AAG) | yfjD → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,747,558 | 0 | A | G | 6.2% | 166.4 / 2.9 | 64 | K13K (AAA→AAG) | yfjD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (28/32); new base G (2/2); total (30/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
CCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGT > W3110S.gb/2747491‑2747626 | ccTATTTTTCCGGTTCAGAAACTGGAATGATGACACTCAACCGCTATCGCCTGAGACACATGGCGAAGCa < 1:4218376/70‑1 (MQ=255)ccTATTTTTCCGGTTCAGAAACTGGAATGATGACACTCAACCGCTATCGCCTGAGACACATGGCGAAGCa < 1:1625872/70‑1 (MQ=255) tttCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTaa > 1:413360/1‑70 (MQ=255) tttCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTaa > 1:2686262/1‑70 (MQ=255) gggTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTc > 1:3509138/1‑71 (MQ=255) ggTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCg > 1:71724/1‑71 (MQ=255) tCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGc > 1:1708477/1‑71 (MQ=255) cGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGcc > 1:4177169/1‑70 (MQ=255) cGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGcc > 1:291878/1‑70 (MQ=255) aaCCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAAc > 1:552765/1‑71 (MQ=255) aaCCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAAc > 1:304389/1‑71 (MQ=255) cGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAAcgcg < 1:1239252/71‑1 (MQ=255) aaTGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTc > 1:378357/1‑70 (MQ=255) atgatgACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCg > 1:1477265/1‑70 (MQ=255) atgaCCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGaaa < 1:2521027/70‑1 (MQ=255) cTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGaaa < 1:648688/64‑1 (MQ=255) cTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGaaa < 1:99429/64‑1 (MQ=255) cTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgct < 1:1013648/71‑1 (MQ=255) cTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGGCGaaa < 1:1794534/64‑1 (MQ=255) tCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGaaa < 1:292201/63‑1 (MQ=255) cAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgctgc > 1:2876169/1‑71 (MQ=255) cAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgctgc > 1:482916/1‑71 (MQ=255) aaCCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgct > 1:2477406/1‑68 (MQ=255) aaCCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCg > 1:3132036/1‑71 (MQ=255) aCCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCa < 1:2350633/48‑1 (MQ=255) ccGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGt > 1:3637976/1‑70 (MQ=255) ccGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTa < 1:451744/71‑1 (MQ=255) cGCTATCGTCTGCGACATATGGCGAGACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTaa < 1:1517256/71‑1 (MQ=255) cGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCaaa > 1:209795/1‑48 (MQ=255) cGCTATCGTCTGCGACATATGGCGAAAAAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTaa < 1:4156207/71‑1 (MQ=255) gCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGaaa < 1:1605973/57‑1 (MQ=255) cTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgctgc < 1:1213321/65‑1 (MQ=255) cTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGc > 1:4049286/1‑71 (MQ=255) cTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGc < 1:966426/71‑1 (MQ=255) cTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGc < 1:2019036/71‑1 (MQ=255) tCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCa < 1:1422268/41‑1 (MQ=255) cGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGaaa < 1:1993000/52‑1 (MQ=255) cGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGa > 1:1647719/1‑71 (MQ=255) cGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGa > 1:2570019/1‑71 (MQ=255) cGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGa > 1:3918556/1‑71 (MQ=255) gccTGAGACACATGGCGAAGCAAGGTAATCGCTCGGCAAAACGCGTCGAAAAATTACTGCGCAAGCCAGAc > 1:3804287/3‑71 (MQ=255) gccTGAGACACATGGCGAAGCAAGGTAATCGCTCGGCAAAACGCGTCGAAAAATTACTGCGCAAGCCAGAc > 1:1102440/3‑71 (MQ=255) gTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCa > 1:2106653/1‑68 (MQ=255) cTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCg > 1:872863/1‑71 (MQ=255) tGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCg < 1:488798/58‑1 (MQ=255) cGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGa > 1:208327/1‑65 (MQ=255) cGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCg < 1:1314939/68‑1 (MQ=255) gACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAg < 1:3073498/59‑1 (MQ=255) gACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAg < 1:1652704/59‑1 (MQ=255) gACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAg < 1:2486472/59‑1 (MQ=255) cATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgctgc < 1:2351533/52‑1 (MQ=255) cATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGAt < 1:4325109/71‑1 (MQ=255) atatGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAg < 1:4102493/60‑1 (MQ=255) atatGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAg < 1:2112346/60‑1 (MQ=255) atatGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGAt > 1:2229418/1‑70 (MQ=255) atatGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGAt > 1:3214558/1‑70 (MQ=255) tatGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGaaa < 1:4368946/41‑1 (MQ=255) tatGGCGAAACAGGGTAATCGCTCGGCCAAACGCGGCGaaaaa < 1:709233/43‑1 (MQ=255) tGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAAt < 1:2514863/42‑1 (MQ=255) tGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAAt < 1:1334456/42‑1 (MQ=255) tGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAAt < 1:2889314/42‑1 (MQ=255) tGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAg < 1:1198206/70‑1 (MQ=255) gCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGa > 1:1839875/1‑56 (MQ=255) gCGAAACAGGGAAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTg > 1:28212/1‑63 (MQ=255) aaaCAGGGTAATCGCTCGGCCAAACGCGTCGAAAAAtt < 1:286097/38‑1 (MQ=255) aaaCAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGgt > 1:16161/1‑71 (MQ=255) aCAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgc > 1:3874115/1‑38 (MQ=255) aCAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgc > 1:4303159/1‑38 (MQ=255) aCAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgc > 1:3095501/1‑38 (MQ=255) aCAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATtgc > 1:242029/1‑38 (MQ=255) | CCTATTTTTCCGGGTCCGAAACCGGAATGATGACCCTCAACCGCTATCGTCTGCGACATATGGCGAAACAGGGTAATCGCTCGGCCAAACGCGTCGAAAAATTGCTGCGTAAGCCAGACCGCCTGATAAGCCTGGT > W3110S.gb/2747491‑2747626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |