| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,089,611 | G→A | 5.6% | P203P (CCG→CCA) | endA → | DNA‑specific endonuclease I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,089,611 | 0 | G | A | 5.6% | 220.5 / 2.0 | 71 | P203P (CCG→CCA) | endA | DNA‑specific endonuclease I |
| Reads supporting (aligned to +/- strand): ref base G (37/30); new base A (2/2); total (39/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
ATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGC > W3110S.gb/3089548‑3089681 | aTACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCg < 1:3983855/71‑1 (MQ=255) tACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGa > 1:825937/1‑71 (MQ=255) tACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGa > 1:3080067/1‑71 (MQ=255) tACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGa < 1:2049972/71‑1 (MQ=255) tACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCAT‑GAACAAGATGTATCCGGTTAc < 1:1845455/67‑1 (MQ=255) aCAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGAc > 1:2763308/1‑71 (MQ=255) cAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCgg > 1:452160/1‑62 (MQ=255) cAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTAcc > 1:3477171/1‑67 (MQ=255) cAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGa > 1:2610654/1‑69 (MQ=255) cAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACt > 1:2095680/1‑71 (MQ=255) aCCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTgg < 1:3864010/71‑1 (MQ=255) aCCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTgg < 1:1765037/71‑1 (MQ=255) cTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTgg > 1:4017225/1‑69 (MQ=255) cacTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGc > 1:3082656/1‑71 (MQ=255) cacTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGc < 1:3172271/71‑1 (MQ=255) ctctctCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTgg < 1:4074476/63‑1 (MQ=255) tctctcGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAg > 1:430947/1‑71 (MQ=255) tctcGCCAGCAAACGCAGCTGTTCAACGCATGGAACATGATGTATCCg < 1:2902996/48‑1 (MQ=255) gCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTAcc > 1:4025130/1‑50 (MQ=255) gCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAgcgc < 1:1113266/68‑1 (MQ=255) ccAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAg < 1:1866466/64‑1 (MQ=255) cAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATg > 1:321727/1‑70 (MQ=255) cAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGa < 1:168276/71‑1 (MQ=255) cAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGa < 1:1570655/71‑1 (MQ=255) aGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGtt < 1:1466277/44‑1 (MQ=255) gCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCg < 1:2042624/47‑1 (MQ=255) gCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATg < 1:405594/68‑1 (MQ=255) aaaCGCAGCTGTTCAACGCATGGGACAAGATGTATCCAGTTACCGACTGGGAGTGCGAGCGTGATGAACGc < 1:996372/71‑1 (MQ=255) aaaCGCAGCTGTTCAACGCATGGGACAAGATGTATCCAGTTACCGACTGGGAGTGCGAGCGTGATGAACGc < 1:1046204/71‑1 (MQ=255) aaaCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGc < 1:3480487/71‑1 (MQ=255) aaCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGt < 1:1008446/53‑1 (MQ=255) aCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt > 1:3535114/1‑71 (MQ=255) aCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt < 1:1222595/71‑1 (MQ=255) aCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt > 1:1655161/1‑71 (MQ=255) aCGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt < 1:3899941/71‑1 (MQ=255) cGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTa > 1:3337847/1‑39 (MQ=255) cGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATc > 1:2638998/1‑71 (MQ=255) cGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATc > 1:1012442/1‑71 (MQ=255) cGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATc > 1:972432/1‑71 (MQ=255) gCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAg > 1:3836594/1‑49 (MQ=255) aGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGa > 1:1590075/1‑58 (MQ=255) gCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:1833156/71‑1 (MQ=255) gCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:2056088/71‑1 (MQ=255) gCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:1315767/71‑1 (MQ=255) cTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATcgcg > 1:1867452/1‑69 (MQ=255) cTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGaa < 1:3292544/71‑1 (MQ=255) tGTTCAACGCATGGAACAAGATGTATCCGGTTAcc < 1:2798683/35‑1 (MQ=255) gTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:4378178/68‑1 (MQ=255) ttCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGt > 1:521523/1‑71 (MQ=255) tCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAg > 1:2395708/1‑68 (MQ=255) cAACGCATGGAACAAGATGTATCCGGTTACCGACTgg < 1:582262/37‑1 (MQ=255) aCGCATGGAACAAGATTTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAg > 1:4212739/1‑71 (MQ=255) tGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCaa < 1:33496/71‑1 (MQ=255) ggAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:1664489/57‑1 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGGGATGAACGCATCGCGaa > 1:330872/1‑57 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt > 1:2883269/1‑51 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCAt > 1:1633014/1‑51 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGc > 1:1759892/1‑62 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:1596620/1‑70 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:4183224/1‑70 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:2091776/1‑70 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:4334473/1‑70 (MQ=255) gAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:3291917/1‑70 (MQ=255) aaCAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGa < 1:3199405/55‑1 (MQ=255) aaCAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATc > 1:824161/1‑70 (MQ=255) aCAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCAt < 1:3423078/71‑1 (MQ=255) cAAGATGTATCCAGTTACCGACTTGGAGTGCGAGCGTGATGAACGCATCGCGAAGGTGCAGGGCAATCAt > 1:3021518/1‑70 (MQ=255) cAAGATGTATCCAGTTACCGACTTGGAGTGCGAGCGTGATGAACGCATCGCGAAGGTGCAGGGCAATCAt > 1:3026408/1‑70 (MQ=255) aGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCAt > 1:4019202/1‑68 (MQ=255) gTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCg > 1:2633123/1‑70 (MQ=255) ccGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAAt > 1:2214662/1‑57 (MQ=255) cGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATaa > 1:2785532/1‑61 (MQ=255) cGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTAtgtg < 1:347980/71‑1 (MQ=255) ggTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCCTATGTGc < 1:472272/71‑1 (MQ=255) | ATACAACCTGACACTCTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAACGCATCGCGAAGGTGCAGGGCAATCATAACCCGTATGTGC > W3110S.gb/3089548‑3089681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |