| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,438,027 | T→G | 6.1% | A313A (GCA→GCC) | hemE ← | uroporphyrinogen decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,438,027 | 0 | T | G | 6.1% | 160.0 / 5.4 | 66 | A313A (GCA→GCC) | hemE | uroporphyrinogen decarboxylase |
| Reads supporting (aligned to +/- strand): ref base T (34/28); new base G (2/2); total (36/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
TTCTGGCGGCACATCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATCCATATTA > W3110S.gb/3437958‑3438096 | ttCTGGCGGCACATCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTg < 1:4031401/71‑1 (MQ=255)ttCTGGCGGCACATCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTg > 1:161867/1‑71 (MQ=255) cTGGCGGCACATCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGc > 1:4056744/1‑70 (MQ=255) gcggcACATCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCa < 1:2631999/68‑1 (MQ=255) cggcACATCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGta > 1:806271/1‑71 (MQ=255) ggcACATCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGta > 1:3987944/1‑70 (MQ=255) ggcACATCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGta > 1:852886/1‑70 (MQ=255) caTCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAg < 1:3931962/64‑1 (MQ=255) caTCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAg < 1:4313944/64‑1 (MQ=255) ccTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGc > 1:2861990/1‑70 (MQ=255) ccTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGc > 1:1302310/1‑70 (MQ=255) cTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCa > 1:731539/1‑58 (MQ=255) cTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTa > 1:1404301/1‑71 (MQ=255) atgaATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTActt > 1:1042354/1‑71 (MQ=255) attccGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTActtcttc < 1:3524083/68‑1 (MQ=255) aTGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCa > 1:1439186/1‑51 (MQ=255) gCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTActtcttctt > 1:1230939/1‑71 (MQ=255) ccGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTActtcttctt > 1:1098740/1‑70 (MQ=255) ccGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACttcttcttc < 1:3212042/71‑1 (MQ=255) ccGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACttcttcttc < 1:1489095/71‑1 (MQ=255) ccGTGACCAAGGCTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACttcttcttc < 1:20699/71‑1 (MQ=255) cGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGta > 1:820943/1‑51 (MQ=255) cGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGta > 1:3155181/1‑51 (MQ=255) tGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACttcttcttc < 1:1762112/68‑1 (MQ=255) aCCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGc > 1:3375882/1‑71 (MQ=255) aCCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGc < 1:3339443/71‑1 (MQ=255) cAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGc < 1:2483687/52‑1 (MQ=255) cAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCgg > 1:3758326/1‑71 (MQ=255) cAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCgg > 1:1118381/1‑71 (MQ=255) ggTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCa < 1:4204204/71‑1 (MQ=255) ggTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCa < 1:2098671/71‑1 (MQ=255) ggTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCCCTACTTCTTCTTCAATGCGGGCa < 1:1684748/71‑1 (MQ=255) gTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGt > 1:45861/1‑45 (MQ=255) gTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAg < 1:943205/71‑1 (MQ=255) aaaGACATGACCTTCGCCGTGACCGAAACCTGCaa > 1:2851401/1‑35 (MQ=255) aaaGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAggcg > 1:4224098/1‑71 (MQ=255) aTGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGt < 1:3004362/71‑1 (MQ=255) tGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTa < 1:4341644/71‑1 (MQ=255) gACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCg > 1:2860385/1‑55 (MQ=255) aCCTTCGCCGTGACCGGAACCTGCAAGTATAGTCGCTAc > 1:4038827/1‑39 (MQ=255) cTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAg > 1:3350643/1‑70 (MQ=255) ttCGCCGTGACCGAAACCTGCAAGTATAGTCGCTActtcttctt < 1:85952/44‑1 (MQ=255) ttCGCCGTGACCGAAACCTGCAAGTATAGTCGCTActtcttctt < 1:3335951/44‑1 (MQ=255) cGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATc > 1:424287/1‑71 (MQ=255) cGCCGTGACCGAAACCGGCAAGTATAGACGCCACTTCTTCTTCAATGc > 1:78840/1‑48 (MQ=38) cGCCGTGACCGAAACCGGCAAGTATAGACGCCACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATc > 1:665061/1‑71 (MQ=255) gCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATc < 1:4099352/70‑1 (MQ=255) gTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACg > 1:1555218/1‑71 (MQ=255) gTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACg > 1:2611551/1‑71 (MQ=255) tGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACg > 1:2936203/1‑70 (MQ=255) gACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGa > 1:3153732/1‑71 (MQ=255) aCCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCa > 1:346364/1‑37 (MQ=255) ccGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATc < 1:3972322/71‑1 (MQ=255) cGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACgg < 1:2604206/67‑1 (MQ=255) cGAAACCGGCAAGTATAGACGCCACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACgg < 1:2672679/67‑1 (MQ=255) cGAAACCGGCAAGTATAGACGCCACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACgg < 1:4240277/67‑1 (MQ=255) gAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGa > 1:1803464/1‑63 (MQ=255) gAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATcc > 1:2346193/1‑70 (MQ=255) gAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATcc > 1:2174533/1‑70 (MQ=255) gAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATCCa < 1:342138/71‑1 (MQ=255) gAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATCCa < 1:1122161/71‑1 (MQ=255) aaCCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATCCata > 1:179318/1‑71 (MQ=255) aCCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATCCatat < 1:865081/71‑1 (MQ=255) aCCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATCCatat < 1:879403/71‑1 (MQ=255) aCCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATCCatat < 1:1064597/71‑1 (MQ=255) cTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATCCATATTa < 1:616334/71‑1 (MQ=255) | TTCTGGCGGCACATCCTGATGAATGCCGTGACCAAGGTTAAAGACATGACCTTCGCCGTGACCGAAACCTGCAAGTATAGTCGCTACTTCTTCTTCAATGCGGGCAGGCGGCGCGTACAGCATCGACGGATCCATATTA > W3110S.gb/3437958‑3438096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |