Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 1,301,041 Δ1,336 bp 100% insCinsD insC, insD

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 1301041–1302370 1302376 7–1336 80 [0] [0] 79 insC–insD insC,insD

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 13010400 (0.000)55 (1.290) 28/84 NT 100% intergenic (+218/‑90) ychE/insC predicted inner membrane protein/IS2 element protein
?W3110S.gb 1302377 = 0 (0.000)intergenic (+19/‑519) insD/oppA IS2 element protein/oligopeptide transporter subunit

CTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1300967‑1301040
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatattaTTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  W3110S.gb/1302377‑1302446
                                                                                                                                                
CTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            <  1:2332973/70‑1
CTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAT                                                                            <  1:2858283/70‑1
   CTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATAT                                                                          <  1:4528492/69‑1
      CTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATT                                                                      <  1:3651251/70‑1
      CTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATT                                                                      <  1:2210810/70‑1
      CTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATT                                                                      >  1:2419740/1‑70
       TCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAA                                                                    >  1:4709272/1‑71
         ACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATT                                                                  >  1:1392529/1‑71
          CCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTC                                                                 <  1:4251495/71‑1
           CATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCC                                                                <  1:3192706/71‑1
           CATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATA                                                                           >  1:909685/1‑60
             TCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTC                                                                 <  1:4711076/68‑1
               ATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACT                                                             >  1:675400/1‑70
                  TTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCT                                                         >  1:1510494/1‑71
                  TTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCT                                                         >  1:4475175/1‑71
                  TTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATT                                                                      <  1:2842995/58‑1
                  TTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATT                                                                      <  1:3464121/58‑1
                   TATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTT                                                        >  1:714971/1‑71
                   TATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCA                                                               >  1:957641/1‑64
                   TATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCA                                                               >  1:4194214/1‑64
                    ATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTT                                                       >  1:3455435/1‑71
                    ATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAA                                                                    >  1:1396663/1‑58
                     TTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATG                                                                              <  1:3020608/47‑1
                      TTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                     >  1:4593476/1‑71
                      TTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                     >  1:4757697/1‑71
                      TTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATG                                                                              <  1:1724052/46‑1
                       TTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                     >  1:4402075/1‑70
                       TTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGG                                                     >  1:2382481/1‑70
                         CGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTA                                                   <  1:2674089/70‑1
                         CGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTA                                                   <  1:1332964/70‑1
                             ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:4285652/71‑1
                             ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:4434280/71‑1
                             ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:3403094/71‑1
                             ACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:336987/71‑1
                              CATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAA                                             <  1:4641714/71‑1
                              CATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAA                                             <  1:3538562/71‑1
                              CATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAA                                             <  1:1170996/71‑1
                              CATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:2155346/70‑1
                              CATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              >  1:385026/1‑70
                                TCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:1867827/68‑1
                                TCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTA                                                                        >  1:1138496/1‑42
                                TCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATA                                                                           >  1:870170/1‑39
                                 CATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:4196499/67‑1
                                 CATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:650293/67‑1
                                 CATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCT                                                         <  1:1874083/56‑1
                                  ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGA                                                 >  1:1782577/1‑63
                                  ATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTT                                                        >  1:2068263/1‑56
                                  ATAATCATGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTG                                         >  1:651/1‑71
                                   TAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTT                                                       <  1:4477415/56‑1
                                    AATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGC                                        >  1:3077196/1‑70
                                    AATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAA                                                                    >  1:3085395/1‑42
                                      TCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      >  1:1863128/1‑70
                                      TCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTA                                      >  1:4416407/1‑70
                                      TCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCAC                                                              >  1:2526220/1‑46
                                      TCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCAC                                                              >  1:2531941/1‑46
                                         TGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGC                                        >  1:4097899/1‑65
                                              TTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAAT                             >  1:3825301/1‑71
                                              TTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAAT                             >  1:3683807/1‑71
                                              TTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTT                                                        <  1:343407/44‑1
                                              TTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTT                                                        <  1:320763/44‑1
                                               TGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTA                                                   <  1:2456555/48‑1
                                                GCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATC                            >  1:3631426/1‑70
                                                  TGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA                                     >  1:1351005/1‑59
                                                  TGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGA                                              <  1:4326524/50‑1
                                                   GAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATT                        >  1:1522865/1‑71
                                                    AAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTG                       >  1:4218490/1‑71
                                                    AAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATT                        <  1:770648/70‑1
                                                       AATAATTGAAATGATATTATTAATTCCACTGCCTT                                                        <  1:1168283/35‑1
                                                       AATAATTGAAATGATATTATTAATTCCACTGCCTT                                                        <  1:3876071/35‑1
                                                          AATTGAAATGATATTATTAATTCCACTGCCTTTGGTAG                                                  <  1:717076/38‑1
                                                           ATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAAT                 >  1:3155904/1‑70
                                                              GAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATT             >  1:4772445/1‑71
                                                               AAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAA                   <  1:4668858/64‑1
                                                               AAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAA                                            >  1:253113/1‑39
                                                                    ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCAT       <  1:4691819/71‑1
                                                                    ATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCAT       <  1:4278850/71‑1
                                                                     TATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATT      >  1:3889432/1‑71
                                                                     TATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCAT       <  1:4808040/70‑1
                                                                       TTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATA                               <  1:4528345/44‑1
                                                                        TATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTG                       <  1:2493771/51‑1
                                                                        TATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTG                       <  1:4026911/51‑1
                                                                         ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:2228229/1‑71
                                                                         ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:4123327/1‑71
                                                                         ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  1:1126592/1‑71
                                                                         ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTC    >  1:1884151/1‑69
                                                                         ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAA                  >  1:794830/1‑55
                                                                         ATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAA                          >  1:1779598/1‑47
                                                                                                                                                
CTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/1300967‑1301040
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gatattaTTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCAC  >  W3110S.gb/1302377‑1302446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.