New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 3982674 =91 (1.830)3 (0.070) 3/84 NT 4.1% intergenic (‑623/+176) gadE/hdeD DNA‑binding transcriptional activator/acid‑resistance membrane protein
?W3110S.gb 3982703 = 61 (1.430)intergenic (‑652/+147) gadE/hdeD DNA‑binding transcriptional activator/acid‑resistance membrane protein
Rejected: Frequency below/above cutoff threshold.

ATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3982750‑3982674
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaattagATGAAATTCAGTAGGTTGAAATAATCACTAGCAGGTAATTATTTCAATGATAGTGCGCAATTGATCTAC  >  W3110S.gb/3982703‑3982771
                                                                                                                                                  
ATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCA                                                                             <  1:1010403/71‑1
 TTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAA                                                                            <  1:669469/71‑1
 TTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCA                                                                             >  1:4442741/1‑70
   GAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAT                                                                          >  1:4444845/1‑71
    AAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATT                                                                         <  1:4274338/71‑1
    AAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATT                                                                         <  1:4176777/71‑1
      ATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAG                                                                       >  1:3307332/1‑71
                GCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATT                                                                         >  1:1436155/1‑59
                     TGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCA                                                                             <  1:4415547/50‑1
                       ATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAT                                                                          <  1:4218381/51‑1
                       ATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAA                                                                           >  1:4324249/1‑50
                           TTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAA                                                                           >  1:4637150/1‑46
                                  CTACTGAATTTCATCTAATTTTTTTCACTCTATGGCA                                                                             <  1:2795207/37‑1
                                   TACTGAATTTCATCTAATTTTTTTCACTCTATGGCA                                                                             <  1:3838215/36‑1
                                            TCATCTAATTTTTTTCACTCTATGGCAAATTAGATGAAATTCAGTAGGTTGAAATAATCACTAGCAGGTAA                                 >  1:3989409/1‑71
                                                    TTTTTTTCACTCTATGGCAAATTAGATGAAATTCAGTAGGTTGAAATAATC                                             <  1:4412015/51‑1
                                                      TTTTTCACTCTATGGCAAATTAGATGAAATTCAGTAGGTTGAAATAATCAC                                           >  1:2191495/1‑51
                                                                            GATGAAATTCAGTAGGTTGAAATAATCACTAGCAGGTAATTATTTCAATGATAGTGCGCAATTGATCTAC  <  1:2313058/70‑1
                                                                                                                                                  
ATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  W3110S.gb/3982750‑3982674
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaattagATGAAATTCAGTAGGTTGAAATAATCACTAGCAGGTAATTATTTCAATGATAGTGCGCAATTGATCTAC  >  W3110S.gb/3982703‑3982771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.