| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,154 | C→T | 18.2% | D606D (GAC→GAT) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,154 | 0 | C | T | 18.2% | 77.6 / 10.7 | 33 | D606D (GAC→GAT) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base C (9/18); new base T (3/3); total (12/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGA > W3110S.gb/2088‑2224 | gtcgATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATAcc < 1:3381117/70‑1 (MQ=255)gtcgATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAcc < 1:3728276/70‑1 (MQ=255) gATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACg > 1:3345745/1‑71 (MQ=255) gATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGATACCAACGTTgg > 1:609236/1‑71 (MQ=255) gATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTg < 1:4506169/70‑1 (MQ=255) ctacCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTg < 1:1838536/71‑1 (MQ=255) cATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGAtt < 1:843735/71‑1 (MQ=255) cATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGAtt < 1:4481460/71‑1 (MQ=255) aGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCg > 1:954291/1‑71 (MQ=255) gCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTa > 1:1245457/1‑71 (MQ=255) gCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTa > 1:1368227/1‑71 (MQ=255) gTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAtt < 1:17185/71‑1 (MQ=255) gTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTAtt < 1:147035/71‑1 (MQ=255) cggcggAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAAc > 1:3577094/1‑71 (MQ=255) gcggAAAAATCGCGGCGTAAATTCCTATAAGACACCAACg < 1:483929/40‑1 (MQ=38) cggAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCg > 1:730806/1‑56 (MQ=255) gAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTgag < 1:2472555/63‑1 (MQ=255) aaaaaTCGCGGCGTAAATTCCTCTATGATACCAACGTTGGGGCTg < 1:886410/45‑1 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGt < 1:3759386/54‑1 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTga > 1:4074287/1‑60 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTga > 1:1620676/1‑60 (MQ=255) aaaaTCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTga > 1:2231810/1‑60 (MQ=255) cgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTa < 1:3172134/46‑1 (MQ=255) cgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTa < 1:1030457/46‑1 (MQ=255) cgTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCaa < 1:3909705/71‑1 (MQ=255) gTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTa < 1:2209594/45‑1 (MQ=255) tAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGtt < 1:4330248/43‑1 (MQ=255) tAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGt < 1:3872416/42‑1 (MQ=255) aaTTCCTCTATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCa < 1:3130319/71‑1 (MQ=255) ctctATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCaa > 1:2525160/1‑62 (MQ=255) tctATGATACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCaa > 1:2413276/1‑61 (MQ=255) tATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaa < 1:4664812/49‑1 (MQ=255) tATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaaa < 1:730463/49‑1 (MQ=255) cacCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATtga < 1:520319/71‑1 (MQ=255) | GTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACACCAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGA > W3110S.gb/2088‑2224 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |