| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 100.0% | 160.6 / NA | 48 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/21); total (27/21) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGG > W3110S.gb/547630‑547760 | gtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:1112150/70‑1 (MQ=255) gcgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAac < 1:3830966/70‑1 (MQ=255) cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc > 1:3314909/1‑71 (MQ=255) cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc > 1:2551099/1‑71 (MQ=255) acTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGcc < 1:1473535/71‑1 (MQ=255) ggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTc > 1:616726/1‑63 (MQ=255) gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc > 1:1684259/1‑49 (MQ=255) gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc > 1:599546/1‑49 (MQ=255) gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCggg < 1:855160/70‑1 (MQ=255) gtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAAt > 1:1523347/1‑71 (MQ=255) tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACg > 1:37819/1‑57 (MQ=255) cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcg > 1:425644/1‑71 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt < 1:863377/71‑1 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt > 1:133246/1‑71 (MQ=255) tcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCa < 1:2074905/57‑1 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAgg < 1:1164403/69‑1 (MQ=255) ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:4677012/1‑67 (MQ=255) aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGa < 1:383911/71‑1 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc < 1:2395281/38‑1 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc < 1:820802/38‑1 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc < 1:3186814/38‑1 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAgg < 1:3578467/63‑1 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCg > 1:275036/1‑40 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa > 1:4768947/1‑49 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:4460821/1‑70 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:2667951/46‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:2521757/46‑1 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAAt > 1:3338431/1‑49 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAAt > 1:2549835/1‑49 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:1902852/1‑57 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:19305/1‑57 (MQ=255) gCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAcc > 1:1906707/1‑70 (MQ=255) cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCa > 1:1970490/1‑71 (MQ=255) cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCa > 1:2591163/1‑71 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg > 1:4625789/1‑70 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:1391501/71‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGgcc > 1:2134421/1‑35 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:3478581/53‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaa < 1:1473704/71‑1 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaa < 1:1198688/71‑1 (MQ=255) aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:4688508/1‑59 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc > 1:219021/1‑46 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGc > 1:3005252/1‑46 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga > 1:548682/1‑52 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaaa < 1:2951707/69‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg > 1:4745434/1‑71 (MQ=255) aCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcg < 1:3225671/40‑1 (MQ=255) aCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAggg < 1:2128771/71‑1 (MQ=255) | GTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGG > W3110S.gb/547630‑547760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |