| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 810,113 | G→A | 27.5% | M116I (ATG→ATA) | bioB → | biotin synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 810,113 | 0 | G | A | 27.5% | 66.9 / 26.4 | 40 | M116I (ATG→ATA) | bioB | biotin synthase |
| Reads supporting (aligned to +/- strand): ref base G (17/12); new base A (4/7); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCT > W3110S.gb/810053‑810176 | cTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAggggg > 1:303089/1‑71 (MQ=255) tGGGCGCGGCGTGGAAGAATCCCCAGGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTaa > 1:1084091/1‑69 (MQ=255) tGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTaa > 1:3089189/1‑69 (MQ=255) tGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTaa > 1:1517825/1‑69 (MQ=255) tGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTaa > 1:2303764/1‑69 (MQ=255) ggCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGc > 1:4642177/1‑71 (MQ=255) cggcgTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATAGTg < 1:829111/53‑1 (MQ=255) tGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTa < 1:2469414/57‑1 (MQ=255) tGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTa < 1:2365619/57‑1 (MQ=255) gaagaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTaaa > 1:2602753/1‑57 (MQ=255) gaagaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTg < 1:1914460/70‑1 (MQ=255) gaagaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTg < 1:2731461/70‑1 (MQ=255) gaagaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTg > 1:805628/1‑70 (MQ=255) gaagaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATAGTGCAGGGGGTAAAAGCGATGGGGCTg > 1:1400568/1‑70 (MQ=255) gaagaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATAGTGCAGGGGGTAAAAGCGATGGGGCTg > 1:3917291/1‑70 (MQ=255) agaaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAg > 1:2047253/1‑71 (MQ=255) aaTCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGt < 1:4766288/50‑1 (MQ=255) ccACGAACGCGATATGCCGTACCTGGAACAAATAGTGCAGGGGGTAAAAGc < 1:4109011/51‑1 (MQ=255) cACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGc < 1:773767/50‑1 (MQ=255) cGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCg < 1:4184007/49‑1 (MQ=255) cGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCg < 1:349755/49‑1 (MQ=255) cgATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTgg > 1:2173063/1‑53 (MQ=255) cgATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTgg > 1:1230199/1‑53 (MQ=255) cgATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTgg > 1:3426617/1‑53 (MQ=255) cgATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTgg > 1:2289836/1‑53 (MQ=255) atGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCAc > 1:528187/1‑71 (MQ=255) atGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCAc > 1:4588281/1‑71 (MQ=255) atGCCGTACCTGGAACAAATAGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCa > 1:4461979/1‑70 (MQ=255) tGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGc < 1:4159720/52‑1 (MQ=255) tGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTg > 1:903731/1‑65 (MQ=255) tGCCGTACCTGGAACAAATAGTGCAGGGGGTAAAAGc < 1:4047265/37‑1 (MQ=255) tGCCGTACCTGGAACAAATAGTGCAGGGGGTAAAAGc < 1:2408150/37‑1 (MQ=255) tGCCGTACCTGGAACAAATAGTGCAGGGGGTAAAAGc < 1:1455639/37‑1 (MQ=255) cGTACCTGGAACAAATAGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGtt > 1:4338993/1‑70 (MQ=255) ccTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGt > 1:589857/1‑70 (MQ=255) tGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCgtgt < 1:1219645/47‑1 (MQ=255) gAACAAATAGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAAt < 1:2934490/70‑1 (MQ=255) aCAAATGGTGCAGCGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAAtct < 1:846769/70‑1 (MQ=255) cAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCgtg < 1:4466738/41‑1 (MQ=255) aaaTAGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTAt < 1:1327052/43‑1 (MQ=255) | CTGTATGGGCGCGGCGTGGAAGAATCCCCACGAACGCGATATGCCGTACCTGGAACAAATGGTGCAGGGGGTAAAAGCGATGGGGCTGGAGGCGTGTATGACGCTGGGCACGTTGAGTGAATCT > W3110S.gb/810053‑810176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |