| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 859,171 | A→G | 7.3% | S105P (TCG→CCG) | ybiU ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 859,171 | 0 | A | G | 7.3% | 148.9 / 4.3 | 55 | S105P (TCG→CCG) | ybiU | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (24/27); new base G (2/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
AGAAATTATCGCCGGGGCCTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGCACAACCGCGACGTTTAAT > W3110S.gb/859105‑859240 | agaaATTATCGCCGGGGCCTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTg < 1:559223/70‑1 (MQ=255)agaaATTATCGCCGGGGCCTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGa < 1:3596858/71‑1 (MQ=255) tATCGCCGGGGCCTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAg > 1:679483/1‑71 (MQ=255) gCCTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCt > 1:436769/1‑70 (MQ=255) cTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCt < 1:805431/68‑1 (MQ=255) cTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCt < 1:3075284/68‑1 (MQ=255) cTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGt < 1:4495054/71‑1 (MQ=255) cTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGt < 1:3494082/71‑1 (MQ=255) tttGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGt > 1:915032/1‑70 (MQ=255) ttGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCtt > 1:3460696/1‑67 (MQ=255) ttGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTc > 1:1189817/1‑71 (MQ=255) tAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTcgc < 1:4134195/70‑1 (MQ=255) tAGACCTCGTCAAAGAGGTTGCGGTCCAGATAGTCCAGCATCGa > 1:3905736/1‑44 (MQ=255) cTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTg < 1:3485705/60‑1 (MQ=255) cTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGa < 1:316466/71‑1 (MQ=255) cTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGa < 1:3103113/71‑1 (MQ=255) cTCGTCAAAGCGGTTGCAGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGa < 1:4753893/71‑1 (MQ=255) tCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTg < 1:2231348/41‑1 (MQ=255) cGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGaaa > 1:3735167/1‑71 (MQ=255) gTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCtt > 1:772140/1‑56 (MQ=255) gTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGaa < 1:1848033/69‑1 (MQ=255) gTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAAt > 1:997855/1‑71 (MQ=255) gTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAAt < 1:2903477/71‑1 (MQ=255) aaaGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCa < 1:669429/42‑1 (MQ=255) aaaGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTg > 1:1289816/1‑54 (MQ=255) aaaGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTg > 1:2821181/1‑54 (MQ=255) aGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCtt < 1:4791281/51‑1 (MQ=255) gCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCggggg > 1:3055435/1‑61 (MQ=255) ttGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTAt < 1:3351670/71‑1 (MQ=255) gCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTcgcg > 1:1800439/1‑51 (MQ=255) tCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGa < 1:1386314/52‑1 (MQ=255) tCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGa < 1:1995131/52‑1 (MQ=255) ccAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCAc > 1:4179081/1‑67 (MQ=255) aGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGcaca < 1:2593587/71‑1 (MQ=255) aGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGcaca < 1:262881/71‑1 (MQ=255) aTAGTCCAGCATCGGCTGATCCCAGCCTAGCGCTTGTTCGCGGGGGaaa < 1:3785764/49‑1 (MQ=255) aTAGTCCAGCATCGGCTGATCCCAGCCTAGCGCTTGTTCGCGGGGGaaa < 1:1205055/49‑1 (MQ=255) aTAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATc < 1:1458081/61‑1 (MQ=255) aGTCCAGCATCGGCTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATg > 1:2404956/1‑49 (MQ=255) aGTCCAGCATCGGCTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATg > 1:1982477/1‑49 (MQ=255) gTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGCACAACcgc < 1:2034273/71‑1 (MQ=255) gTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGCACAACcgc < 1:2654175/71‑1 (MQ=255) cAGCATCGACTGATCCCAGCCTAGCGCTTGTTcgcg > 1:1602281/1‑36 (MQ=255) cAGCATCGACTGATCCCAGCCTAGCGCTTGTTcgcg > 1:3100876/1‑36 (MQ=255) cAGCATCGACTGATCCCAGCCTAGCGCTTGTTcgc > 1:634905/1‑35 (MQ=255) cAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCTGGGGAAATGGCCTTTTATCACCGCACAACCGCGAc > 1:1187159/1‑71 (MQ=255) cAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGaaa > 1:4262002/1‑43 (MQ=255) cAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGaaa > 1:2753890/1‑43 (MQ=255) cAGCATCGACTGATCCCAGCATAGCGCTTGTTCGCGGGGGAAATGGCCTTTTAt > 1:3761084/1‑54 (MQ=255) cAGCATCGACTGATCCCAGACTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGCACAACCGCGa > 1:3440287/1‑70 (MQ=255) aGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCttt < 1:3123951/50‑1 (MQ=255) tCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTAt < 1:4612047/49‑1 (MQ=255) tCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTAt < 1:2061182/49‑1 (MQ=255) gACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGCACAACCGCGACGTTTaa > 1:2437527/1‑70 (MQ=255) gACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGCACAACCGCGACGTTTAAt > 1:450636/1‑71 (MQ=255) gACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGCACAACCGCGACGTTTAAt > 1:1539773/1‑71 (MQ=255) | AGAAATTATCGCCGGGGCCTTTGTAGACCTCGTCAAAGCGGTTGCGGTCCAGATAGTCCAGCATCGACTGATCCCAGCCTAGCGCTTGTTCGCGGGGGAAATGGCCTTTTATCACCGCACAACCGCGACGTTTAAT > W3110S.gb/859105‑859240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |