Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,633 G→T 100% Q246Y (CAG→TAT oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6330GT100.0% 154.4 / NA 51Q246Y (CAG→TAToppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/18);  total (33/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT  >  W3110S.gb/1303568‑1303695
                                                                 |                                                              
aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                           >  1:3849127/1‑71 (MQ=255)
    aacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                       >  1:2008660/1‑71 (MQ=255)
    aacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                       >  1:1213162/1‑71 (MQ=255)
         aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                  <  1:10140/71‑1 (MQ=255)
         aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                  <  1:2692839/71‑1 (MQ=255)
         aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                  <  1:2609609/71‑1 (MQ=255)
         aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                  <  1:1073465/71‑1 (MQ=255)
               cTTGAACGCAGCCCGACCTACTGGAACAACTCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:1534053/1‑71 (MQ=255)
               cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:4456399/1‑71 (MQ=255)
               cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:3008344/1‑71 (MQ=255)
               cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:3566273/1‑71 (MQ=255)
               cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:4007867/1‑71 (MQ=255)
                   aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                         <  1:3059511/70‑1 (MQ=255)
                      gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                     <  1:1343832/71‑1 (MQ=255)
                      gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                     <  1:1914154/71‑1 (MQ=255)
                       cAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                    >  1:3936379/1‑71 (MQ=255)
                       cAGCCCGACCTACTGGAACAACGCGAAAAACGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                    >  1:2562352/1‑71 (MQ=255)
                         gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt                                                 <  1:1922995/56‑1 (MQ=255)
                         gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt                                   >  1:4363381/1‑70 (MQ=255)
                          cccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                           >  1:3928784/1‑45 (MQ=38)
                           ccGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                          >  1:1223511/1‑45 (MQ=38)
                             gACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                     <  1:3149503/64‑1 (MQ=255)
                              aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                      >  1:1647206/1‑46 (MQ=39)
                              aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                      >  1:2225866/1‑46 (MQ=39)
                                cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg                           >  1:1616544/1‑71 (MQ=255)
                                 tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:2717348/41‑1 (MQ=38)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:754451/1‑51 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:1676783/1‑51 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                               <  1:3123141/64‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc                               <  1:1161419/64‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                         <  1:3432293/70‑1 (MQ=255)
                                   cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                         >  1:372845/1‑70 (MQ=255)
                                   cTGGAACAACGCGAAAACCGATATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:1347460/1‑51 (MQ=255)
                                    tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa                       >  1:2944830/1‑71 (MQ=255)
                                    tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGACa                        >  1:4320045/1‑70 (MQ=255)
                                     ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                      >  1:643521/1‑71 (MQ=255)
                                     ggAAAAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                      >  1:420700/1‑71 (MQ=255)
                                      gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                      >  1:2050473/1‑70 (MQ=255)
                                        acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                      >  1:3007294/1‑52 (MQ=255)
                                        acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTCACCGATGt                          >  1:4014963/1‑64 (MQ=255)
                                         caacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt                            >  1:4362345/1‑61 (MQ=255)
                                         caacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCt                  <  1:1687497/71‑1 (MQ=255)
                                         caacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCt                  <  1:1118142/71‑1 (MQ=255)
                                         caacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCCTCTGAAGTTACCGAt                            >  1:4490459/1‑61 (MQ=255)
                                            cgcgAAAACCGTTATTAACTATGTAACCTATTTGc                                                   >  1:398295/1‑35 (MQ=37)
                                            cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAcc               >  1:3969062/1‑71 (MQ=255)
                                              cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt             <  1:1209112/71‑1 (MQ=255)
                                              cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt             <  1:4367472/71‑1 (MQ=255)
                                                aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                      <  1:2069257/44‑1 (MQ=38)
                                                         attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt  >  1:1964491/1‑71 (MQ=255)
                                                           taACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                             >  1:2414269/1‑42 (MQ=38)
                                                                 |                                                              
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT  >  W3110S.gb/1303568‑1303695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: