Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,058,034 C→T 25.3% G279G (GGC→GGT amn → AMP nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,058,0340CT25.3% 134.9 / 46.0 71G279G (GGC→GGTamnAMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (26/27);  new base T (12/6);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

AAAAACATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTTGATGA  >  W3110S.gb/2057964‑2058104
                                                                      |                                                                      
aaaaaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGc                                                                        <  1:1443783/71‑1 (MQ=255)
 aaaaCATCAGATGCCAGCATGGCATTTAATTACAGCCGATGGTCAGGGTATTACTCTGGTGATTATTGGCg                                                                       >  1:3318928/1‑71 (MQ=255)
  aaaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGt                                                                      <  1:3063030/71‑1 (MQ=255)
   aaCATCAGATGCCAGCATGGCATTTAATTACCGGCGATGGTCAGGGTATTACTCTGGTGAATATTGGtgtg                                                                     >  1:1479677/1‑71 (MQ=255)
   aaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGtgtg                                                                     >  1:483108/1‑71 (MQ=255)
   aaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTg                                                                     >  1:2318592/1‑71 (MQ=255)
   aaCATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTg                                                                     >  1:2457610/1‑71 (MQ=255)
      aTCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGa                                                                  <  1:4083266/71‑1 (MQ=255)
            tGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCa                                                            <  1:3015253/71‑1 (MQ=255)
            tGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCa                                                            <  1:966811/71‑1 (MQ=255)
            tGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCa                                                            <  1:2438761/71‑1 (MQ=255)
             gCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCa                                                            >  1:1116789/1‑70 (MQ=255)
              ccAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaa                                                           <  1:1185249/70‑1 (MQ=255)
                  cATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGc                                                       >  1:4261054/1‑70 (MQ=255)
                   aTGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATg                                                        >  1:134861/1‑68 (MQ=255)
                   aTGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTa                                                     <  1:3909651/71‑1 (MQ=255)
                   aTGGCAATTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATg                                                        >  1:1469/1‑68 (MQ=255)
                        aTTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAAcc                                                <  1:3348787/71‑1 (MQ=255)
                         tttAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCa                                                            <  1:3412218/58‑1 (MQ=255)
                         tttAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAAcc                                                <  1:2135779/70‑1 (MQ=255)
                         tttAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAAcc                                                <  1:1200807/70‑1 (MQ=255)
                         tttAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAAcc                                                <  1:4477504/70‑1 (MQ=255)
                          ttAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCAt                                              >  1:4014228/1‑71 (MQ=255)
                           tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCAt                                              >  1:3193735/1‑70 (MQ=255)
                           tAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATc                                             >  1:935461/1‑71 (MQ=255)
                            aaTTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGa                                                                  >  1:2829666/1‑49 (MQ=255)
                              ttACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGgtgt                                                                      <  1:2598135/43‑1 (MQ=255)
                                aCCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGAcc                                                                >  1:853794/1‑47 (MQ=255)
                                aCCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGAcc                                                                >  1:513991/1‑47 (MQ=255)
                                 ccgccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCaa                                                           >  1:612801/1‑51 (MQ=255)
                                  cgccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCa                                                            <  1:1126525/49‑1 (MQ=255)
                                   tccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGt                                                                      <  1:1402436/37‑1 (MQ=255)
                                   gccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATc                                             >  1:4197616/1‑63 (MQ=255)
                                    ccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCa                                                            <  1:3506426/47‑1 (MQ=255)
                                    ccgATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGatcat                                    >  1:1279470/1‑71 (MQ=255)
                                    ccgATGGTCAGGGTATTACTCTGGTGAATATTCGCGTGGGACCGTCa                                                            <  1:4354843/47‑1 (MQ=255)
                                       aTGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTg                                 >  1:4292369/1‑71 (MQ=255)
                                        tGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTc                                                             >  1:4539443/1‑42 (MQ=255)
                                        tGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg                                <  1:1521439/71‑1 (MQ=255)
                                        tGGTCAGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg                                <  1:4432920/71‑1 (MQ=255)
                                         ggTCTGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg                                >  1:816491/1‑70 (MQ=255)
                                         ggTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg                                >  1:4477531/1‑70 (MQ=255)
                                             aGGGTATTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGa                                        >  1:3804945/1‑58 (MQ=255)
                                             aGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAg                             >  1:2952967/1‑69 (MQ=255)
                                             aGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTg                           >  1:3552910/1‑71 (MQ=255)
                                             aGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTg                           >  1:1547278/1‑71 (MQ=255)
                                                gTATTACTCTGGTGAATATTGGCGTGGGACCGTCa                                                            <  1:4461521/35‑1 (MQ=255)
                                                  aTTACTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCt                         <  1:768599/68‑1 (MQ=255)
                                                  aTTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTAc                       >  1:1783065/1‑70 (MQ=255)
                                                   ttACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCt                         <  1:3825521/67‑1 (MQ=255)
                                                     aCTCTGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCa                              >  1:469781/1‑60 (MQ=255)
                                                     aCTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCa                              >  1:4592455/1‑60 (MQ=255)
                                                     aCTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc                   >  1:2291553/1‑71 (MQ=255)
                                                     aCTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGccc                   >  1:1073464/1‑71 (MQ=255)
                                                         tGGTGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATc                                             <  1:3442248/41‑1 (MQ=255)
                                                         tGGTGAATATTGGCGTGCGACCGTCAAATGCTAAAAcc                                                <  1:2274093/38‑1 (MQ=255)
                                                           gTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTa                        <  1:2864481/60‑1 (MQ=255)
                                                           gTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGAt               <  1:2620619/69‑1 (MQ=255)
                                                            tGAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGt             <  1:645639/70‑1 (MQ=255)
                                                             gAATATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGatcatc                                   >  1:4606207/1‑47 (MQ=255)
                                                             gAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGat                                       >  1:3389217/1‑43 (MQ=255)
                                                             gAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCt           >  1:2188762/1‑71 (MQ=255)
                                                              aaTATTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCt           >  1:580537/1‑70 (MQ=255)
                                                              aaTATTGGCGTGGGACCGTCAAATGCTAAAACCATc                                             >  1:53269/1‑36 (MQ=255)
                                                               atatTGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTgg         <  1:3294394/71‑1 (MQ=255)
                                                                tatTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTg          >  1:3461358/1‑69 (MQ=255)
                                                                  ttGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGtt       >  1:2308588/1‑70 (MQ=255)
                                                                  ttGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGtt       >  1:2661612/1‑70 (MQ=255)
                                                                  ttGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGc                               <  1:3453274/46‑1 (MQ=255)
                                                                  ttGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGc                               <  1:1103859/46‑1 (MQ=255)
                                                                   tGGTGTGGGACCGTCAAATGCTAAAACCATCTGCGat                                       >  1:4131540/1‑37 (MQ=255)
                                                                   tGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg                                <  1:2985335/44‑1 (MQ=255)
                                                                   tGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg                                <  1:2931449/44‑1 (MQ=255)
                                                                   tGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTgg                                <  1:3804194/44‑1 (MQ=255)
                                                                     gCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTtgat    <  1:3310428/70‑1 (MQ=255)
                                                                      cGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGTCAGTGCTACGCCCGGATGTCTGGTtgatga  <  1:1781907/71‑1 (MQ=255)
                                                                      |                                                                      
AAAAACATCAGATGCCAGCATGGCATTTAATTACCGCCGATGGTCAGGGTATTACTCTGGTGAATATTGGCGTGGGACCGTCAAATGCTAAAACCATCTGCGATCATCTGGCAGTGCTACGCCCGGATGTCTGGTTGATGA  >  W3110S.gb/2057964‑2058104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: