| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,809,581 | G→A | 49.3% | G52G (GGG→GGA) | mprA → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,809,581 | 0 | G | A | 49.3% | 11.1 / 222.9 | 144 | G52G (GGG→GGA) | mprA | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (35/38); new base A (37/34); total (72/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
Only 100 of 149 total aligned reads displayed.
CTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTG > W3110S.gb/2809514‑2809651 | gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc > 1:3988984/1‑71 (MQ=255) gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc < 1:1537103/71‑1 (MQ=255) gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc < 1:3200826/71‑1 (MQ=255) gTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTaa < 1:3426854/70‑1 (MQ=255) tCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc < 1:3514844/70‑1 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc > 1:1607052/1‑68 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTa > 1:556286/1‑64 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACgaga < 1:862066/70‑1 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGAc > 1:1891236/1‑71 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAATGCTCAGGGAATTAACGAGACgttgtt < 1:4552688/71‑1 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt < 1:4097929/71‑1 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgtt > 1:966284/1‑71 (MQ=255) catgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTa > 1:2261253/1‑71 (MQ=255) catgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa < 1:346496/71‑1 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt < 1:70731/71‑1 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt > 1:1931995/1‑71 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt > 1:1910318/1‑71 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc < 1:1267337/56‑1 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgtt > 1:3351225/1‑64 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgtt > 1:1293273/1‑64 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgtt > 1:4076305/1‑64 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATg > 1:2744960/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTa > 1:4503291/1‑52 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:1867974/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:3769974/1‑71 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt < 1:2109365/65‑1 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt > 1:751866/1‑71 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGtt < 1:2899108/71‑1 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt < 1:3132562/70‑1 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg < 1:3656111/71‑1 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGtt > 1:416768/1‑70 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGt > 1:2176124/1‑69 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:1790565/71‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:3416860/71‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:1298280/71‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTaa > 1:329413/1‑47 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTaa > 1:1049683/1‑47 (MQ=255) agcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt < 1:3252910/57‑1 (MQ=255) agcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTa > 1:1119812/1‑45 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTg < 1:857593/67‑1 (MQ=255) ctgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAc < 1:1996262/71‑1 (MQ=255) tgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTa > 1:769270/1‑69 (MQ=255) ctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt > 1:1610033/1‑53 (MQ=255) ctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg < 1:2073254/69‑1 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTa > 1:2971573/1‑66 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:1090315/38‑1 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:1929841/38‑1 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:2503832/38‑1 (MQ=255) tgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACga < 1:481124/43‑1 (MQ=255) ggAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1202894/1‑53 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:388534/1‑69 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa < 1:1953496/71‑1 (MQ=255) agaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTg > 1:2851509/1‑59 (MQ=255) aCCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt > 1:2867250/1‑46 (MQ=255) aCCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:4291982/58‑1 (MQ=255) cGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgt < 1:1411143/40‑1 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGAc > 1:2314053/1‑37 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgtt > 1:3385897/1‑40 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg > 1:631678/1‑71 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt < 1:2638786/51‑1 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt < 1:3929107/51‑1 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACgaga > 1:4150878/1‑35 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:411976/1‑42 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc < 1:3487692/46‑1 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa > 1:4573848/1‑61 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:3687532/71‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:1303813/1‑50 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg < 1:4067862/61‑1 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACgttgtt > 1:2013130/1‑37 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACgttgtt > 1:583254/1‑37 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACgttgt < 1:2734190/36‑1 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa > 1:3400169/1‑48 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAc > 1:4318268/1‑71 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:3072635/1‑39 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGTAGTCTCAGGAAAAc < 1:514482/71‑1 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAc > 1:4384266/1‑71 (MQ=255) aaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTgg > 1:1683888/1‑56 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTa > 1:2892593/1‑49 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca < 1:3523750/71‑1 (MQ=255) aTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca < 1:1000849/71‑1 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt > 1:2928460/1‑41 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg > 1:1503991/1‑42 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct < 1:601052/57‑1 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct < 1:3369409/57‑1 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg < 1:3733130/71‑1 (MQ=255) aaGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa > 1:2703599/1‑40 (MQ=255) aaGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTa < 1:398821/70‑1 (MQ=255) aaGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAt > 1:4672727/1‑71 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGt > 1:4725791/1‑52 (MQ=255) ggCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAc < 1:4649864/61‑1 (MQ=255) ggCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAc < 1:1645102/61‑1 (MQ=255) ggCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAc < 1:2080899/61‑1 (MQ=255) gCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCa < 1:4612693/71‑1 (MQ=255) cTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaaa > 1:1394359/1‑58 (MQ=255) cTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTAAGGAAAACca > 1:1825541/1‑61 (MQ=255) tCATGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAg < 1:1483466/70‑1 (MQ=255) tCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct < 1:2266131/49‑1 (MQ=255) aGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGcc < 1:926265/70‑1 (MQ=255) gggaATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCtt > 1:443134/1‑71 (MQ=255) gATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTg < 1:1569725/71‑1 (MQ=255) | CTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTG > W3110S.gb/2809514‑2809651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |