| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,866,110 | T→A | 100% | intergenic (‑20/+160) | rpoS ← / ← nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,866,110 | 0 | T | A | 100.0% | 202.6 / NA | 67 | intergenic (‑20/+160) | rpoS/nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (33/34); total (33/34) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATT > W3110S.gb/2866044‑2866174 | aaCACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc > 1:3793725/1‑71 (MQ=255) acacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctac > 1:4525412/1‑71 (MQ=255) acacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctac > 1:1940110/1‑71 (MQ=255) acacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGACAAATCGTTATCACTGGGTTCCAATTctac > 1:119839/1‑71 (MQ=255) cacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:4245069/71‑1 (MQ=255) cacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:586190/71‑1 (MQ=255) cacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:3723001/71‑1 (MQ=255) gCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAg < 1:1680936/71‑1 (MQ=255) gCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAg < 1:3759402/71‑1 (MQ=255) cTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAg < 1:1449433/70‑1 (MQ=255) tgtgtgGCTCCCTGCGATAACAGTTCCTCTTCGGTCAAATCGTTATCACTGGGTTCCAATTctacta < 1:3423621/67‑1 (MQ=255) tgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCttt > 1:1657167/1‑71 (MQ=255) tgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCttt > 1:2858496/1‑71 (MQ=255) tgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCttt > 1:2819021/1‑71 (MQ=255) tCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGt > 1:4434721/1‑71 (MQ=255) ccTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:629314/56‑1 (MQ=255) tGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaa > 1:3570846/1‑70 (MQ=255) tGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaa > 1:3475342/1‑70 (MQ=255) tGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTAGTCaa > 1:2791186/1‑70 (MQ=255) gCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa > 1:3664535/1‑70 (MQ=255) gCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa > 1:3170659/1‑70 (MQ=255) gCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa > 1:702422/1‑70 (MQ=255) cGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctac < 1:2419187/51‑1 (MQ=255) cGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:3611194/69‑1 (MQ=255) aaCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctacta > 1:4235016/1‑49 (MQ=255) aaCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:105073/1‑46 (MQ=255) aaCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:402250/1‑46 (MQ=255) aaCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAg > 1:3185955/1‑51 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:2857475/64‑1 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:2810484/64‑1 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTc < 1:1064952/70‑1 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTc < 1:695120/70‑1 (MQ=255) cAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:394342/1‑44 (MQ=255) aGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctacta > 1:4169525/1‑46 (MQ=255) aGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAAc > 1:2233058/1‑71 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:4296107/1‑36 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg < 1:1084278/70‑1 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGt > 1:4795687/1‑71 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:2808535/39‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:2285093/35‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:3283497/35‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAg < 1:1624404/42‑1 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtttt < 1:2992929/47‑1 (MQ=255) ggCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtca < 1:2328095/70‑1 (MQ=255) ggCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtca < 1:4462195/70‑1 (MQ=255) ccAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:760512/71‑1 (MQ=255) cAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca > 1:2104003/1‑70 (MQ=255) cAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatc > 1:1866515/1‑69 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCt < 1:421173/52‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCt < 1:67795/52‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCaaa < 1:4246752/71‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCaaa < 1:1052425/71‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCaa > 1:702288/1‑70 (MQ=255) aTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:3736796/45‑1 (MQ=255) aTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg > 1:4035109/1‑58 (MQ=255) aTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAtt < 1:2608152/71‑1 (MQ=255) cGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTc < 1:801721/49‑1 (MQ=255) cGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCGGTTCtcatca < 1:3538769/65‑1 (MQ=255) cGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTcc > 1:4035394/1‑71 (MQ=255) cGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc > 1:3303645/1‑70 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCaa > 1:3047614/1‑50 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg < 1:39833/71‑1 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg < 1:2213476/71‑1 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg > 1:1117934/1‑71 (MQ=255) gTTATCACTGGGTTCAAATTCTACTAAGGCCTTTTCGTCAAAAACCTCaa > 1:1280618/1‑50 (MQ=255) ttATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:643776/63‑1 (MQ=255) ggTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCAtt > 1:3924079/1‑71 (MQ=255) | AACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATT > W3110S.gb/2866044‑2866174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |