| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,053,471 | A→G | 28.4% | F9S (TTT→TCT) | pepP ← | proline aminopeptidase P II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,053,471 | 0 | A | G | 28.4% | 66.6 / 34.7 | 47 | F9S (TTT→TCT) | pepP | proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base A (11/22); new base G (8/5); total (19/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01 | |||||||||||
TGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGT > W3110S.gb/3053404‑3053533 | tGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAAct < 1:1711723/71‑1 (MQ=255) cagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAActct < 1:3800460/71‑1 (MQ=255) cagcaAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAActct > 1:1592691/1‑71 (MQ=255) aaaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGcc > 1:4637555/1‑71 (MQ=255) aaaaaTCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGcc > 1:3456131/1‑71 (MQ=255) gcggcgCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCtt < 1:1236565/58‑1 (MQ=255) gcggcgCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCtt < 1:3203678/58‑1 (MQ=255) gcggcgCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATAtct > 1:4201751/1‑70 (MQ=255) gcgcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGaaa > 1:4250872/1‑50 (MQ=255) gcgcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATctcact > 1:2106505/1‑71 (MQ=255) gcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCggg > 1:103290/1‑59 (MQ=255) cTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCAt < 1:4077075/71‑1 (MQ=255) cTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCAt < 1:434373/71‑1 (MQ=255) tGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCAt > 1:644661/1‑70 (MQ=255) gCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATaa > 1:3434758/1‑71 (MQ=255) ttGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCgg < 1:733157/49‑1 (MQ=255) gCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGa > 1:3471002/1‑49 (MQ=255) tttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCgg < 1:2597382/44‑1 (MQ=255) tttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCAt < 1:2919962/58‑1 (MQ=255) tttCCTCCACCATGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCAt < 1:209920/58‑1 (MQ=255) ttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCgg < 1:308273/43‑1 (MQ=255) ttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTa > 1:3444310/1‑70 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACg > 1:3731593/1‑71 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAaca > 1:2615433/1‑60 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAaca > 1:3058595/1‑60 (MQ=255) tGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAaca > 1:859230/1‑60 (MQ=255) cTCCACCAGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtt < 1:2722581/71‑1 (MQ=255) tCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCt < 1:649309/64‑1 (MQ=255) caGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATctcactca < 1:2263086/47‑1 (MQ=255) caGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttt < 1:4435024/67‑1 (MQ=255) caGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttt < 1:140624/67‑1 (MQ=255) caGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGt < 1:1386768/71‑1 (MQ=255) caGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGt < 1:4187512/71‑1 (MQ=255) aGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATctcactc > 1:3878927/1‑45 (MQ=255) aGGGCCTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGtt > 1:3878919/1‑71 (MQ=255) aGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCtt > 1:4574794/1‑59 (MQ=255) aGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttt < 1:730476/66‑1 (MQ=255) gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGttttt < 1:1384933/71‑1 (MQ=255) ccTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGttttt < 1:4395774/70‑1 (MQ=255) cTGACGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGtttt > 1:2411232/1‑68 (MQ=255) tGACGGCGACGCTGAGACTCTTGCCGGGATATctcactca < 1:3648436/40‑1 (MQ=255) tGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttt < 1:1199105/60‑1 (MQ=255) tGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttt < 1:3710309/60‑1 (MQ=255) aCGGCGACGCTGATACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGt < 1:2381641/62‑1 (MQ=255) aCGGCGACGCTGAGACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttttt < 1:1000211/60‑1 (MQ=255) gACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTCTTTGTTTTTAGTGTagagg < 1:3138385/71‑2 (MQ=255) aCGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttttt < 1:4412524/54‑1 (MQ=255) cTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGtttt > 1:3982930/1‑49 (MQ=255) | TGCAGCAAAAATCAGCGCGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGT > W3110S.gb/3053404‑3053533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |