| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,152,234 | G→A | 26.9% | D6N (GAT→AAT) | yghB → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,152,234 | 0 | G | A | 26.9% | 139.1 / 55.3 | 78 | D6N (GAT→AAT) | yghB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (28/29); new base A (9/12); total (37/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01 | |||||||||||
TTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTT > W3110S.gb/3152165‑3152303 | ttCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa < 1:2864333/71‑1 (MQ=255) cAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGat < 1:3790381/70‑1 (MQ=255) cAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGat < 1:2596218/70‑1 (MQ=255) cAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGat < 1:1656952/70‑1 (MQ=255) aGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcat > 1:2382956/1‑71 (MQ=255) aGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcat > 1:1391355/1‑71 (MQ=255) cAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGc > 1:3199593/1‑70 (MQ=255) aaTAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa < 1:3163703/60‑1 (MQ=255) aTAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgc > 1:1491770/1‑71 (MQ=255) tAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatca > 1:3123607/1‑63 (MQ=255) tAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgcg > 1:1035728/1‑71 (MQ=255) ggCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGct > 1:2796520/1‑71 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcat < 1:3025371/57‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatc < 1:3847470/55‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGc < 1:181614/71‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGc < 1:2143954/71‑1 (MQ=255) tAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGc < 1:4769018/70‑1 (MQ=255) tAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGc > 1:2162360/1‑70 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc > 1:2519501/1‑58 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc > 1:1291089/1‑58 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:1125350/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:938842/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:1140102/1‑71 (MQ=255) ggCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAacac < 1:1129887/71‑1 (MQ=255) gCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCaa > 1:4005755/1‑67 (MQ=255) ttAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa > 1:249594/1‑43 (MQ=255) ttAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa > 1:665353/1‑43 (MQ=255) ttAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcatc > 1:2348490/1‑50 (MQ=255) ttAAACGCTGTTACACAGGAAAGTCCATGGATGTTATTCAAGATatcatc > 1:2689817/1‑50 (MQ=255) tAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatc < 1:3361487/46‑1 (MQ=255) aaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAacac < 1:4499372/65‑1 (MQ=255) aaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACttt < 1:3565152/71‑1 (MQ=255) aaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACttt < 1:3120010/71‑1 (MQ=255) aaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACttt < 1:898450/71‑1 (MQ=255) aCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACttt > 1:4426438/1‑70 (MQ=255) tGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCg < 1:3146970/43‑1 (MQ=255) tCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt > 1:174312/1‑71 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt < 1:3568291/70‑1 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt < 1:2569805/70‑1 (MQ=255) aGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCgg < 1:2320292/71‑1 (MQ=255) ggAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGa < 1:808884/71‑1 (MQ=255) ggAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGa < 1:4491625/71‑1 (MQ=255) aaaGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATc > 1:2705516/1‑71 (MQ=255) aaaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATc > 1:2195572/1‑71 (MQ=255) aaaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATc > 1:4164781/1‑71 (MQ=255) aaaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATc > 1:244733/1‑71 (MQ=255) aGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCt < 1:944159/71‑1 (MQ=255) gTCCATGGCTGTTATTCAAAATATCATCGCTGCGctct < 1:3798289/38‑1 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGctc < 1:3578029/36‑1 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc > 1:241989/1‑58 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTc > 1:3564742/1‑70 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCa < 1:1134164/71‑1 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCa > 1:4068058/1‑71 (MQ=255) tCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACttt < 1:3370988/51‑1 (MQ=255) tCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACttt < 1:3885475/51‑1 (MQ=255) cATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGa < 1:3873819/45‑1 (MQ=255) cATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCata < 1:1077682/71‑1 (MQ=255) cATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACttt > 1:284845/1‑49 (MQ=255) cATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACttt > 1:3715270/1‑49 (MQ=255) aTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGa < 1:2500380/44‑1 (MQ=255) aTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCcgc < 1:4486063/53‑1 (MQ=255) aTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCcgc < 1:2290036/53‑1 (MQ=255) ggCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTg > 1:753445/1‑71 (MQ=255) gCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGt < 1:85237/71‑1 (MQ=255) cTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGtt < 1:4398843/71‑1 (MQ=255) gTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAg > 1:4074318/1‑71 (MQ=255) ttATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGc < 1:1022955/71‑1 (MQ=255) tATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCg > 1:4651141/1‑71 (MQ=255) tATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCg > 1:2481341/1‑71 (MQ=255) aTTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc > 1:861173/1‑46 (MQ=255) aTTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgt > 1:2641138/1‑71 (MQ=255) aTTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgt > 1:4786224/1‑71 (MQ=255) ttCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGtt < 1:1586317/65‑1 (MQ=255) ttCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgtt < 1:4072629/71‑1 (MQ=255) ttCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgtt < 1:1308122/71‑1 (MQ=255) cAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAg > 1:1631181/1‑65 (MQ=255) cAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgttg < 1:4807942/70‑1 (MQ=255) aGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGttt < 1:807490/71‑1 (MQ=255) | TTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTT > W3110S.gb/3152165‑3152303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |