| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,315,139 | T→A | 27.0% | D244V (GAT→GTT) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,315,139 | 0 | T | A | 27.0% | 100.7 / 37.9 | 59 | D244V (GAT→GTT) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base T (22/21); new base A (5/11); total (27/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAG > W3110S.gb/3315071‑3315205 | tgttgCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACg > 1:2007345/1‑71 (MQ=255) gttgCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGc > 1:479489/1‑71 (MQ=255) gttgCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGA‑CACGGCCACGGCCGCCTTCGACTTCACGATCGCt > 1:2740660/1‑71 (MQ=255) gCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCttt > 1:3393934/1‑71 (MQ=255) gCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCtt > 1:4701588/1‑70 (MQ=255) tcttcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGtct > 1:3252607/1‑71 (MQ=255) cGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGtcttct < 1:1778460/70‑1 (MQ=255) ctgctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTc < 1:3512559/55‑1 (MQ=255) ctgctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTc < 1:3506797/55‑1 (MQ=255) ctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCa > 1:3088797/1‑71 (MQ=255) ctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCa > 1:421836/1‑71 (MQ=255) tCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATg < 1:2872160/70‑1 (MQ=255) cgcgTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGGGCATGtt > 1:3869779/1‑71 (MQ=255) cgcgTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGt > 1:2189097/1‑50 (MQ=255) gcgTTACGACCTCGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTg < 1:3107840/71‑1 (MQ=255) gcgTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGtt > 1:4519627/1‑70 (MQ=255) cgTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCa < 1:436932/65‑1 (MQ=255) gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:1956235/71‑1 (MQ=255) gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:3720215/71‑1 (MQ=255) ttACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCtt < 1:2133196/42‑1 (MQ=255) tACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGtctt > 1:118328/1‑48 (MQ=255) aCGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTATTCTGCCTGGCGAGCATGTTGAGAag > 1:4143146/1‑71 (MQ=255) aCGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGa > 1:1650567/1‑58 (MQ=255) cGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCttt > 1:4561664/1‑40 (MQ=255) cGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCttt > 1:590660/1‑40 (MQ=255) cGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagt < 1:1292450/71‑1 (MQ=255) ccacggccacggCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagtagt < 1:4155192/71‑1 (MQ=255) cacggccacggcCGCCTTCGACTTCACGAACGCTTTCGtc < 1:347611/40‑1 (MQ=255) cggccacggccGCCTTCGACTTCACGATCGCTTTc < 1:3630628/35‑1 (MQ=255) ggccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGAc > 1:3245778/1‑70 (MQ=255) ggccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACg > 1:4186334/1‑71 (MQ=255) gccacggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:997337/59‑1 (MQ=255) gccacggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:2631034/59‑1 (MQ=255) cacggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGCCGTGa > 1:4306674/1‑71 (MQ=255) cggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATa < 1:4423616/71‑1 (MQ=255) ggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCa < 1:3396286/47‑1 (MQ=255) gccgccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagta > 1:4775665/1‑58 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgaga > 1:4655079/1‑48 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGAc > 1:3251654/1‑57 (MQ=255) tCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTAGAGAAGTAGTGAc > 1:2382029/1‑56 (MQ=255) cGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCa > 1:1859400/1‑38 (MQ=255) cGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGaa > 1:1165167/1‑71 (MQ=255) cGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGa < 1:676893/70‑1 (MQ=255) gACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGa > 1:1252423/1‑58 (MQ=255) gACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAAt < 1:781176/71‑1 (MQ=255) cTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCt < 1:1781305/71‑1 (MQ=255) ttCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgaga < 1:2657846/42‑1 (MQ=255) ttCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGa < 1:738129/55‑1 (MQ=255) ttCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGa < 1:1105665/55‑1 (MQ=255) tCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCg < 1:1436806/60‑1 (MQ=255) cACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCtt < 1:2404010/69‑1 (MQ=255) aCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGTTAATCGc > 1:1775776/1‑59 (MQ=255) aCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAg < 1:82048/71‑1 (MQ=255) cGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:4354334/37‑1 (MQ=255) cGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:855521/37‑1 (MQ=255) gATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATa < 1:4667004/52‑1 (MQ=255) aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagtagt < 1:2590553/42‑1 (MQ=255) aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagtagt < 1:2644539/42‑1 (MQ=255) aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAg < 1:1509650/68‑1 (MQ=255) | TGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAG > W3110S.gb/3315071‑3315205 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |