| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,688,001 | A→G | 40.0% | S440G (AGT→GGT) | yifB → | predicted bifunctional protein, enzyme and transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,688,001 | 0 | A | G | 40.0% | 37.5 / 70.2 | 60 | S440G (AGT→GGT) | yifB | predicted bifunctional protein, enzyme and transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (17/19); new base G (16/8); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGAT > W3110S.gb/3687932‑3688068 | aaaCAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAg > 1:2760543/1‑71 (MQ=255) aacGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCg < 1:2521794/71‑1 (MQ=255) cgcgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATa > 1:482845/1‑64 (MQ=255) cgTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGaaa < 1:135852/71‑1 (MQ=255) tAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGaaa < 1:4096580/69‑1 (MQ=255) aTGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATag > 1:2766827/1‑69 (MQ=255) tGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACg < 1:4730690/70‑1 (MQ=255) tGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGc > 1:818002/1‑71 (MQ=255) ggCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGc > 1:117982/1‑70 (MQ=255) gccgccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGcc < 1:262680/70‑1 (MQ=255) gccgccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGcc < 1:1323151/70‑1 (MQ=255) gccgccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGcc < 1:2744131/70‑1 (MQ=255) gccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAAtt < 1:4446910/71‑1 (MQ=255) ccAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGa < 1:333683/58‑1 (MQ=255) agagagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAAtt < 1:152825/68‑1 (MQ=255) gagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCaa < 1:4570235/71‑1 (MQ=255) gagCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCa > 1:2230429/1‑70 (MQ=255) ccAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTg < 1:1701311/71‑1 (MQ=255) cAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTg < 1:697553/70‑1 (MQ=255) aTTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTc > 1:4061511/1‑58 (MQ=255) aTTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:3878103/1‑69 (MQ=255) tttAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:4698452/1‑68 (MQ=255) ttAAGCGGCAGAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGagag > 1:2983790/1‑70 (MQ=255) tAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCTATTCTGCAAACTTGAGAGc > 1:3950502/1‑70 (MQ=255) aGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGaa < 1:3231009/71‑1 (MQ=255) cGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg > 1:996407/1‑67 (MQ=255) gAATAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGa > 1:3493928/1‑71 (MQ=255) gAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAAc < 1:1372497/53‑1 (MQ=255) gAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTg < 1:3232423/56‑1 (MQ=255) gAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGa > 1:1148145/1‑71 (MQ=255) aTAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACtt < 1:2192248/53‑1 (MQ=255) aTAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg > 1:3080967/1‑60 (MQ=255) aTAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg > 1:4502958/1‑60 (MQ=255) aTAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCg > 1:3881898/1‑60 (MQ=255) aTAAACTGAATGCCTGGCTGGATAGTCCGGAAATAc > 1:3111110/1‑36 (MQ=255) tAAACTGAATGCCTGGCTGGATGGTCCGGAAATACGCAAATTCTGCAAACTTGAGAGCGAAGATGCGAtgt > 1:2003694/1‑71 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAAtt > 1:3063023/1‑42 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCg < 1:2445221/67‑1 (MQ=255) tAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGAtgt > 1:2233729/1‑71 (MQ=255) aaaCTGAATGCCTGGCTGGATGGTCCGGAAATACGc > 1:850825/1‑36 (MQ=255) aaaCTGAATGCCTGGCTGGATGGTCCGGAAATACGc > 1:1530504/1‑36 (MQ=255) aCTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTga > 1:1050961/1‑51 (MQ=255) gAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGaa < 1:89006/55‑1 (MQ=255) aaTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTgg < 1:3603015/70‑1 (MQ=255) aaTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTgg < 1:4687335/70‑1 (MQ=255) aaTGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATg > 1:3362564/1‑58 (MQ=255) aaTGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATg > 1:3234059/1‑58 (MQ=255) aTGCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTgg > 1:4703415/1‑69 (MQ=255) aTGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGa > 1:1839419/1‑55 (MQ=255) tGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaag < 1:2547858/71‑1 (MQ=255) gCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGGAAACTTGAGAGCGaa > 1:2744348/1‑51 (MQ=39) gCCTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGaa > 1:1765114/1‑51 (MQ=255) cccggctggATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg > 1:417345/4‑70 (MQ=255) ccTGGCTGGATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGt > 1:1608127/1‑63 (MQ=255) ccTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaagg < 1:3190305/70‑1 (MQ=255) ctggctggATGGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGa > 1:1892389/1‑51 (MQ=255) ctggctggATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGaa < 1:822821/49‑1 (MQ=255) tggctggATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTggaaggaa < 1:1584824/70‑1 (MQ=255) gATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTg < 1:1888636/69‑1 (MQ=255) gATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTg < 1:2138186/69‑1 (MQ=255) gATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGAt > 1:4730766/1‑71 (MQ=255) | AAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTGGCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGAT > W3110S.gb/3687932‑3688068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |