| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,175 | A→G | 38.6% | R263R (CGA→CGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,175 | 0 | A | G | 38.6% | 65.4 / 102.4 | 88 | R263R (CGA→CGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (23/31); new base G (23/11); total (46/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGAT > W3110S.gb/71107‑71244 | accaCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGt < 1:89503/68‑1 (MQ=255) ccaCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa > 1:18092/3‑71 (MQ=255) cGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCTACTCTATTTCTCGCGGGTAttt > 1:2649341/1‑69 (MQ=255) gATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:315489/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaa < 1:672981/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaa < 1:4416985/71‑1 (MQ=255) aTGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaa < 1:2582080/71‑1 (MQ=255) ccTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATg < 1:1601586/71‑1 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGa > 1:1567387/1‑56 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTa < 1:934605/59‑1 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:4709571/66‑1 (MQ=255) cTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGc < 1:532053/71‑1 (MQ=255) tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCa > 1:4338335/1‑71 (MQ=255) tATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAg < 1:1718033/56‑1 (MQ=255) aTCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaaa > 1:1872605/1‑66 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCTCGAg > 1:499527/1‑54 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaaa > 1:2810166/1‑65 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCAcc > 1:3821445/1‑71 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCAcc > 1:1483422/1‑71 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCAcc > 1:518609/1‑71 (MQ=255) tCGCCACCGTCGGTCGCAATGTTGGTTTCGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:409182/1‑65 (MQ=255) cGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCg > 1:131168/1‑71 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCgg > 1:3910242/1‑71 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCg < 1:17722/70‑1 (MQ=255) gCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCg < 1:1428944/70‑1 (MQ=255) ccACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCggg < 1:916908/71‑1 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc > 1:3366597/1‑71 (MQ=255) aCCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGc < 1:783707/71‑1 (MQ=255) ccGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCAcc > 1:518969/1‑65 (MQ=255) ccGTCGGTCGCAATGTTGCTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGc > 1:4500255/1‑70 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCa > 1:2090460/1‑61 (MQ=255) gtcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATCTaaaaaa > 1:23877/1‑57 (MQ=255) tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:1835459/54‑1 (MQ=255) tcggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAAt > 1:1831227/1‑57 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGa > 1:4666891/1‑42 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa < 1:529657/52‑1 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGccc < 1:2873389/71‑1 (MQ=255) ggtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGccc > 1:1188895/1‑71 (MQ=255) gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaa < 1:3619163/50‑1 (MQ=255) gtcgCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcg < 1:1801306/71‑1 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:1379441/1‑71 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:3335285/1‑71 (MQ=255) gCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:3690989/1‑71 (MQ=255) cAATGTTGGTTTTGACGCTCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:2173135/1‑70 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:2098847/1‑70 (MQ=255) cAATGTTGGTTTTGACGATCAACTCTATTTCTAGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagcg < 1:3370073/71‑1 (MQ=255) aaTGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:2190293/1‑69 (MQ=255) aaTGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaa < 1:3701945/44‑1 (MQ=255) aaTGTTGGTTTTGAAGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcgagc > 1:2032297/1‑69 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgggg < 1:2799732/56‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaaaa < 1:47516/46‑1 (MQ=255) tGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa > 1:3267914/1‑44 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:1209190/1‑44 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaa > 1:2530672/1‑42 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCcga > 1:2547443/1‑63 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:3015093/42‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCcgagcg < 1:4097132/66‑1 (MQ=255) ttGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGttt < 1:1448620/71‑1 (MQ=255) tGGTTTTGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:4632314/1‑43 (MQ=255) tttGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGc > 1:2797073/1‑71 (MQ=255) ttGACGATCAACTCTATTTCTCGCGGGTATTTaaaaaa > 1:3759226/1‑38 (MQ=255) ttGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGcc > 1:1876837/1‑71 (MQ=255) tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATg < 1:1811369/39‑1 (MQ=255) tGACGATCAACTCTATTTCTCGCGGGTATTTAAAAAATGAACCGGGGCCAGCCCGAGCGAGTTCCGTGCCg > 1:1018471/1‑71 (MQ=255) tGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:1350282/35‑1 (MQ=255) tGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:2338937/35‑1 (MQ=255) tGACGATCAACTCTATTTCTCGCGAGTATTTaaaa < 1:1986259/35‑1 (MQ=255) gATCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtg < 1:4276213/71‑1 (MQ=255) aTCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCgg > 1:80778/1‑40 (MQ=255) aTCAACTCTATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgt < 1:497713/71‑1 (MQ=255) cAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCgg > 1:3481128/1‑65 (MQ=255) cAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgtg > 1:2282029/1‑70 (MQ=255) cAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTtgtg > 1:2714218/1‑70 (MQ=255) ctctATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaag > 1:1371123/1‑70 (MQ=255) ctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTtgt < 1:4423142/64‑1 (MQ=255) ctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGTTGTgaagaa > 1:1655515/1‑70 (MQ=255) ctATTTCTCGCGGGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTCCGTGCCGGATGTgaagaa > 1:549452/1‑70 (MQ=255) tATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaagaa < 1:4392900/69‑1 (MQ=255) aTTTCTCGAGAGTATTTAAAAAATGCACCGGGGCCAg > 1:3950722/1‑37 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaaa < 1:159269/70‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaa < 1:2494383/69‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGaaaa < 1:1549652/69‑1 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAg > 1:1936441/1‑71 (MQ=255) tttCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAg > 1:3868679/1‑71 (MQ=255) tctcGGGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGt < 1:351640/70‑1 (MQ=255) ctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTgaag > 1:1635575/1‑62 (MQ=255) ctcGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTg < 1:1839003/70‑1 (MQ=255) gAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:2153724/71‑1 (MQ=255) gAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatgat < 1:649374/71‑1 (MQ=255) gAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAatga < 1:654882/70‑1 (MQ=255) | ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAAGTGAATGAT > W3110S.gb/71107‑71244 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |