| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,195,173 | A→G | 25.0% | *118* (TAA→TGA) | rplR → | 50S ribosomal subunit protein L18 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,195,173 | 0 | A | G | 25.0% | 30.5 / 9.7 | 20 | *118* (TAA→TGA) | rplR | 50S ribosomal subunit protein L18 |
| Reads supporting (aligned to +/- strand): ref base A (4/11); new base G (3/2); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGGTTCCAATATCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGAACTGCAGGAAAAGCTGATCGC > W3110S.gb/4195104‑4195238 | ccGTGTTCCAATATCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTa < 1:1702309/70‑1 (MQ=255) gTTCCAATATCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTa < 1:2509590/71‑1 (MQ=255) tatCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGgtgt < 1:751168/71‑1 (MQ=255) atCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGgtgt > 1:58469/1‑70 (MQ=255) tCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTaa < 1:413345/71‑1 (MQ=255) tCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTagag < 1:1229128/59‑1 (MQ=255) tCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGACTTCAGTTCTAAGGTagag < 1:233695/59‑1 (MQ=255) cAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTagag > 1:858865/1‑52 (MQ=255) aCTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGaaaa < 1:831580/71‑1 (MQ=255) cTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATgg < 1:1664180/57‑1 (MQ=255) ggCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAAc > 1:1625046/1‑70 (MQ=255) ggCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATc > 1:1952934/1‑63 (MQ=255) gCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACa < 1:1903889/70‑1 (MQ=255) cAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACaa < 1:1300935/70‑1 (MQ=255) gctgcCCGTGAAGCTGGCCTTCAGTTCTGAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGc > 1:1889255/1‑71 (MQ=255) gctgcCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGc > 1:115545/1‑71 (MQ=255) tgcCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGa < 1:278089/56‑1 (MQ=255) aaGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAg > 1:1075164/1‑56 (MQ=255) gCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAAc < 1:1646764/47‑1 (MQ=255) gTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGAACTGCAGGAAAAGCTGATcgc < 1:63852/71‑1 (MQ=255) tCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGAACTGCAGGAAAAGCTg < 1:1742106/64‑1 (MQ=255) | CCGGGTTCCAATATCATGGTCGTGTCCAGGCACTGGCAGATGCTGCCCGTGAAGCTGGCCTTCAGTTCTAAGGTAGAGGTGTAAGATGGCTCACATCGAAAAACAAGCTGGCGAACTGCAGGAAAAGCTGATCGC > W3110S.gb/4195104‑4195238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |