Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	643,492	T→G	A245A (GCA→GCC)	rna ←	ribonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	643,492	0	T	G	100.0%	65.6 / NA	35	A245A (GCA→GCC)	rna	ribonuclease I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (0/35);  total (0/35)

AGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGC  >  W3110S.gb/643471‑643506                      |               tggTTGTGGCATGAATGAGTTGGCAGAAAGCGGAGc  <  1:338687/35‑1 (MQ=37) tggTTGTGGCAATAATGAGTTGGCAGAAAGCGGAGc  <  1:120487/35‑1 (MQ=37) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:32997/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:213932/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:2148/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:240267/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:254590/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:263/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:272243/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:272626/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:312320/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:206421/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:337566/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:34639/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:35772/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:59601/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:69933/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:76420/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:146570/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:10597/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:113596/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:117853/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:12148/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:129733/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:131354/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:134957/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:105192/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:154021/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:154049/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:170117/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:1908/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:196833/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:201142/35‑1 (MQ=38) tggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:205035/35‑1 (MQ=38)  ggTTGTGGCAAGAATGAGTTGGCAGAAAGCGGAGc  <  1:5070/35‑1 (MQ=255)                      |               AGGTTGTGGCAAGAATGAGTTTGCAGAAAGCGGAGC  >  W3110S.gb/643471‑643506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑