Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 1,308,595 G→C A90A (GCC→GCG)  yciU ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,308,5950GC100.0% 1306.3 / NA 722A90A (GCC→GCG) yciUhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (0/722);  total (0/722)

Only 100 of 723 total aligned reads displayed.

TTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCC  >  W3110S.gb/1308571‑1308631
                        |                                    
tttttCGCGTCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:421/61‑1 (MQ=255)
cttttCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:246148/60‑1 (MQ=255)
    tagcgGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:243128/55‑1 (MQ=255)
    tCGCTGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:180485/57‑1 (MQ=255)
    tCGCTGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:94221/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:293253/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:326195/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:322203/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:317109/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:31230/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:310563/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:306413/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:300747/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:299543/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:296660/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:294688/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:33112/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:289412/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:286534/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:28504/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:283157/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:271611/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:268696/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:266909/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:100414/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:255263/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:51093/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:97270/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:90705/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:88879/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:85257/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:77952/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:7587/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:72772/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:66885/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:60767/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:57169/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:52862/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:328792/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:47642/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:40149/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:37412/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:34684/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:343774/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:339512/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:338636/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:336322/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:33264/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:257330/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:137431/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:177662/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:25405/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:172152/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:169503/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:163628/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:160529/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:156830/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:153540/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:14989/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:146276/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:141446/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:176625/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:135608/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:132899/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:129534/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:126892/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:123817/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:121784/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:120279/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:113732/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:110665/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:109022/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:107828/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:220661/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:251236/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:239205/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:236259/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:233995/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:228351/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:224756/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:222869/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:184476/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:216667/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:210161/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:20309/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:200703/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:198641/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:189845/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:195859/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:194051/57‑1 (MQ=255)
    tCGCGGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTca  <  1:8168/57‑2 (MQ=255)
    tCGCGACATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:213980/57‑1 (MQ=255)
     cgcgGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:324372/56‑1 (MQ=255)
     cgcgGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:261943/56‑1 (MQ=255)
     cgcgGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:6947/56‑1 (MQ=255)
     cgcgGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:205476/56‑1 (MQ=255)
     cgcgGCATAACAGAATGCGCGCGAAAACATCGTTGATATCACCATCTTCACTGTcc  <  1:118498/56‑1 (MQ=255)
       cgGCATAACAGAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:263501/54‑1 (MQ=255)
                 gAATGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:274934/44‑1 (MQ=38)
                  aaTGCGCGCGAAAACATCGTTGATTTCACCATCTTCACTGTcc  <  1:155297/43‑1 (MQ=38)
                        |                                    
TTTTTCGCGGCATAACAGAATGCGGGCAAAAACATCGTTGATTTCACCATCTTCACTGTCC  >  W3110S.gb/1308571‑1308631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑