Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,435,079	A→G	R202R (CGT→CGC)	purF ←	amidophosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,435,079	0	A	G	100.0%	25.5 / NA	15	R202R (CGT→CGC)	purF	amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/15);  total (0/15)

TATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGG  >  W3110S.gb/2435071‑2435117         |                                       tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:1785/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:190575/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:194975/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:20198/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:228880/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:274461/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:301536/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:312186/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:6242/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:66848/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:79843/47‑1 (MQ=255) tatTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:80832/47‑1 (MQ=255) tatTCTGTGCGGGTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:226009/47‑1 (MQ=255)  atTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:252913/46‑1 (MQ=255)  atTCTGTGCGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCgg  <  1:316135/46‑1 (MQ=255)         |                                       TATTCTGTACGGTTCTCGTCAATATCACGTTTTCCCAGTACCAGCGG  >  W3110S.gb/2435071‑2435117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑