Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 2,538,196 | G→C | T114T (ACG→ACC) | cysK → | cysteine synthase A, O‑acetylserine sulfhydrolase A subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,538,196 | 0 | G | C | 99.7% | 653.8 / NA | 312 | T114T (ACG→ACC) | cysK | cysteine synthase A, O‑acetylserine sulfhydrolase A subunit |
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (311/0); minor base A (1/0); total (312/0) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 312 total aligned reads displayed.
CCGCAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACGGA > W3110S.gb/2538154‑2538198 | ccgcAAGCTGCTGAAAGCGTTAGGTGCAACCCTGGTGCTGACCGa > 1:244117/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:285498/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:336113/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:331476/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:32748/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:314925/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:311150/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:308043/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:304943/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:301032/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:297396/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:2936/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:289524/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:287789/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:33939/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:284786/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:283602/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:280758/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:2779/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:276615/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:272379/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:27007/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:264938/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:261035/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:26000/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:257700/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:60527/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:97144/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:91777/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:88404/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:85872/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:83282/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:82569/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:78245/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:73460/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:70331/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:68655/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:649/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:64282/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:252903/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:58415/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:54022/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:50438/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:48492/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:4510/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:42359/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:38171/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:348048/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:34673/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:343420/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:341096/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:133196/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:17533/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:171838/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:168603/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:167050/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:162375/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:156662/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:150410/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:145752/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:144210/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:140954/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:137636/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:178812/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:128595/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:127623/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:125060/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:12159/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:119405/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:117213/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:114063/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:108630/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:107445/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:101908/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:101075/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:216181/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:251712/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:249193/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:247769/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:239292/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:237531/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:235256/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:232119/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:22561/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:222243/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:218132/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:217031/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:100678/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:212783/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:21054/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:206416/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:20238/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:2011/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:196239/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:195129/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:189788/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:187468/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:185070/1‑45 (MQ=255) ccgcAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:183666/1‑45 (MQ=255) ccgcAAGCTGATGAAAGCGTTAGGTGCAAACCTGGTGCTGACCGa > 1:131710/1‑45 (MQ=38) | CCGCAAGCTGCTGAAAGCGTTAGGTGCAAACCTGGTGCTGACGGA > W3110S.gb/2538154‑2538198 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |