Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,830,691 C→A V85V (GTG→GTT)  norR ← DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,830,6910CA100.0% 939.5 / NA 495V85V (GTG→GTT) norRDNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/495);  total (0/495)

Only 100 of 495 total aligned reads displayed.

GTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTC  >  W3110S.gb/2830673‑2830735
                  |                                            
gTCTGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCtt   <  1:127580/62‑1 (MQ=255)
   tGCTGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:204356/60‑1 (MQ=255)
   tGCGGGAAAGCGCTCAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:155156/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCtta  <  1:872/60‑2 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:318872/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:323976/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:314903/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:310532/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:308973/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:304918/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:299747/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:297477/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:292932/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:289919/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:285823/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:281327/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:279838/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:273305/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:268924/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:268341/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:262586/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:256933/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:254070/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:251314/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:246392/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:100836/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:48433/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:94786/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:9340/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:90488/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:82972/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:78687/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:73428/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:70938/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:64897/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:62237/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:58078/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:53050/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:320409/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:45461/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:41438/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:38402/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:343688/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:338425/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:336775/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:333132/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:329919/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:242458/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:321235/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:126550/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:169918/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:238093/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:152983/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:14943/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:146571/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:144019/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:142271/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:138415/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:134526/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:132508/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:130058/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:167818/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:124538/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:118945/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:117092/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:1153/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:111456/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:109716/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:107131/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:104453/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:10399/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:102896/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:206194/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:236062/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:233802/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:232260/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:229521/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:225095/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:221590/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:215997/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:209413/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:174212/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:203665/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:2026/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:177021/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:198607/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:191941/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:179603/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:183160/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:189248/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:186833/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCGTCCAGCCGTGGATGCCCTTc  <  1:162979/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCACTTCCAGCCGGGGATGCCCTTc  <  1:27722/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGCGGGCAATAGCTTCCAGCCGTGGATGCCCTTc  <  1:282221/60‑1 (MQ=255)
   tGCGGGAAAGCGCACAACATCACCCGCGAGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:36742/60‑1 (MQ=255)
    gCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:2123/59‑1 (MQ=255)
    gCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATCCCCTTc  <  1:158489/59‑1 (MQ=255)
    gCGGGAAAGCGCACAACATCACCCGCGCGGGCAATCGCGTCCAGCCGTGGATGCCCTTc  <  1:243885/59‑1 (MQ=255)
       ggAAAGCGCACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:121108/56‑1 (MQ=255)
              gcACAACATCACCCGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTc  <  1:68154/49‑1 (MQ=38)
                  |                                            
GTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTC  >  W3110S.gb/2830673‑2830735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑