| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 332,518 | A→G | L308L (CTA→CTG) | yahA → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 332,518 | 0 | A | G | 99.8% | 905.7 / NA | 504 | L308L (CTA→CTG) | yahA | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (503/0); minor base T (1/0); total (504/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
Only 100 of 504 total aligned reads displayed.
CAAATGGCGAGTGTCGACGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTAGCGC > W3110S.gb/332462‑332522 | cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:292706/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:330798/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:326683/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:322579/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:319407/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:317471/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:31444/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:310292/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:307403/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:305521/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:301605/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:297598/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:295757/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:336258/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:288626/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:282245/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:280579/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:277263/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:275450/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:272648/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:269821/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:266879/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:264527/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:262/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:259392/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:63248/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:974/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:95880/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:94579/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:91185/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:87794/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:85507/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:80994/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:79433/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:75669/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:7120/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:68112/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:65626/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:254660/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:59837/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:5700/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:55931/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:52845/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:46671/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:45544/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:4274/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:37575/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:35541/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:345315/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:343404/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:144704/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:177308/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:17482/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:172189/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:170510/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:166556/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:161609/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:159862/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:157591/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:154702/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:151469/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:150259/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:147521/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:179705/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:139407/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:134238/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:131211/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:126332/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:118880/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:118060/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:115908/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:113790/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:112511/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:108077/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:103948/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:217285/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:253141/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:249061/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:246893/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:240477/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:238510/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:236333/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:234949/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:230965/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:229325/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:225435/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:223440/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:219176/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:100206/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:212675/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:209107/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:205777/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:201704/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:199046/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:196599/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:194044/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:192318/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:188394/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:186180/1‑61 (MQ=255)cAAATGGCGAGTGTCGATGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTGgcgc > 1:18156/1‑61 (MQ=255) | CAAATGGCGAGTGTCGACGAAATCTCCGGTCATATTGTGGACAATATTGTCGAACTAGCGC > W3110S.gb/332462‑332522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |