Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,972,249 C→T L90L (CTG→CTA)  ygeD ← predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,972,2490CT100.0% 1273.8 / NA 673L90L (CTG→CTA) ygeDpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (673/0);  total (673/0)

Only 100 of 673 total aligned reads displayed.

CCCAGCAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCACCT  >  W3110S.gb/2972244‑2972307
     |                                                          
cccAGTAGCTTCAGTCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:48479/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCAc                               >  1:225137/1‑35 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGc              >  1:28583/1‑52 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGcccc    >  1:79089/1‑60 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCa     >  1:322557/1‑61 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCa     >  1:252840/1‑61 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:327188/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:271795/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:319100/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:316487/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:313948/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:309779/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:306372/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:301316/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:297363/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:294465/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:292454/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:2897/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:288806/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:260512/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:266252/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:284586/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:282499/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:280011/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:277686/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:268052/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:275788/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:58861/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:95995/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:92732/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:89576/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:85385/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:8398/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:80904/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:75730/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:68467/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:65454/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:63953/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:60764/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:258167/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:55811/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:51913/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:44099/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:3946/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:36680/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:344836/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:338509/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:337156/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:331627/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:330284/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:142113/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:177685/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:100252/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:17201/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:169334/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:167484/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:162836/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:161355/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:158370/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:151768/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:147686/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:145569/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:175016/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:137124/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:133836/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:129757/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:125995/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:12189/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:120591/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:118209/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:115989/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:114099/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:109625/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:103994/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:218958/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:2511/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:247548/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:245451/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:242560/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:2407/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:238257/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:235029/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:232255/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:230089/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:221829/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:179722/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:217646/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:21248/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:210413/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:207167/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:205002/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:2024/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:197931/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:193180/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:187661/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:185629/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:18324/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCATTTGGCGAAGCTATCCGCCAc    >  1:341712/1‑62 (MQ=255)
cccAGTAGCTTCAGGCCGTTGGCAAACATCATCA‑‑CGGCCTTTGGCGAAGCTATCCGCCACCt  >  1:70998/1‑62 (MQ=255)
cccAGTAGCTTAAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCAc    >  1:106718/1‑62 (MQ=255)
     |                                                          
CCCAGCAGCTTCAGGCCGTTGGCAAACATCATCACCCGGCCTTTGGCGAAGCTATCCGCCACCT  >  W3110S.gb/2972244‑2972307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑