Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,498,881 T→G intergenic (+132/‑143) gldA → / → yijF glycerol dehydrogenase, NAD/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,498,8810TG100.0% 918.8 / NA 515intergenic (+132/‑143)gldA/yijFglycerol dehydrogenase, NAD/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (515/0);  total (515/0)

Only 100 of 515 total aligned reads displayed.

GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA  >  W3110S.gb/3498872‑3498933
         |                                                    
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAACCTTCCa               >  1:118892/1‑49 (MQ=21)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTTCACCTTGTTCgggtg  >  1:145582/1‑60 (MQ=21)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCCCCTTGTTCgggtg  >  1:317539/1‑60 (MQ=21)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACcttgtt        >  1:167532/1‑56 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACcttgt         >  1:152743/1‑55 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACctt           >  1:2809/1‑53 (MQ=21)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:333/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:340517/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:330291/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:328280/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:326982/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:323711/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:321509/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:3129/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:307696/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:299501/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:294804/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:29251/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:288117/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:28554/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:283281/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:277214/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:275456/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:273286/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:270263/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:26645/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:261447/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:55241/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:96782/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:92326/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:86496/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:84200/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:81149/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:75872/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:72233/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:66387/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:6519/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:60374/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:58432/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:336851/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:53279/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:50760/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:48911/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:44793/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:41622/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:37566/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:348644/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:343365/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:109097/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:338132/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:148456/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:197111/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:195624/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:190552/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:186898/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:183777/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:182729/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:172191/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:162584/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:161086/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:157471/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:149922/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:193931/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:139931/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:137012/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:134784/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:132664/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:130969/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:127566/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:121364/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:115019/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:113346/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:100527/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:227091/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:253722/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:252454/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:248886/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:246474/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:245273/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:242837/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:241160/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:238438/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:234124/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:232389/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:199744/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:225666/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:220625/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:215597/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:21311/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:211275/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:208217/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:2051/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt   >  1:179345/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt   >  1:125864/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt   >  1:95189/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTACCACCTTGTTCgggtg  >  1:302772/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTCAAACTTCCACcttgtt        >  1:310322/1‑56 (MQ=21)
ggCGTTCACGCCGCATCCGGCACGCTGTGCAAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:25895/1‑60 (MQ=21)
ggCGTTCACGCCGCATCCGGCAAGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:263320/1‑60 (MQ=18)
ggCGTTCACGCCGCATACGGCACGCTGTGCCAGTAATCTGAAACtt                  >  1:174928/1‑45 (MQ=25)
         |                                                    
GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA  >  W3110S.gb/3498872‑3498933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑