Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,498,917 C→T intergenic (+168/‑107) gldA → / → yijF glycerol dehydrogenase, NAD/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,498,9170CT99.6% 941.6 / NA 505intergenic (+168/‑107)gldA/yijFglycerol dehydrogenase, NAD/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (503/0);  minor base A (2/0);  total (505/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 506 total aligned reads displayed.

GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA  >  W3110S.gb/3498872‑3498933
                                             |                
ggCGTTCCCGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCCCCTTGTTCgggtg  >  1:1460/1‑60 (MQ=19)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACcttgtt        >  1:167532/1‑56 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACcttgt         >  1:152743/1‑55 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTc       >  1:246783/1‑57 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:330291/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:338132/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:328326/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:327370/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:324022/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:322111/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:318820/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:3129/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:307696/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:299501/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:294804/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:29251/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:288117/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:28554/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:283689/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:281678/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:277513/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:276274/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:273492/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:27033/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:268258/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:264379/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:261593/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:55267/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:96782/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:92326/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:86496/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:84200/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:81149/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:77045/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:72313/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:67152/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:65356/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:60401/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:58647/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:333/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:53930/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:51836/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:49065/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:44793/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:41360/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:37263/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:347745/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:343365/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:340517/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:260373/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:336851/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:14259/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:188365/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:185860/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:183416/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:179514/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:176400/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:172191/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:163180/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:161399/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:157811/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:149922/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:148456/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:190583/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:13794/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:135285/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:132664/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:130969/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:127566/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:125994/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:122448/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:119061/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:115019/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:113346/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:109097/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:223685/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:253722/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:252454/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:248886/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:245278/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:243369/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:2416/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:239483/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:234243/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:232541/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:230219/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:226632/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:100527/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:218570/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:213725/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:211819/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:20852/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:205432/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:197361/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:195821/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:194029/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt   >  1:20095/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt   >  1:95189/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTACCACCTTGTTCgggtg  >  1:302772/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTCAAACTTCCACcttgtt        >  1:310322/1‑56 (MQ=21)
                                             |                
GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA  >  W3110S.gb/3498872‑3498933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: