Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 3,498,917 | C→T | intergenic (+168/‑107) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,498,917 | 0 | C | T | 99.6% | 941.6 / NA | 505 | intergenic (+168/‑107) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (503/0); minor base A (2/0); total (505/0) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 506 total aligned reads displayed.
GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA > W3110S.gb/3498872‑3498933 | ggCGTTCCCGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCCCCTTGTTCgggtg > 1:1460/1‑60 (MQ=19) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACcttgtt > 1:167532/1‑56 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACcttgt > 1:152743/1‑55 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTc > 1:246783/1‑57 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:330291/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:338132/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:328326/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:327370/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:324022/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:322111/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:318820/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:3129/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:307696/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:299501/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:294804/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:29251/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:288117/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:28554/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:283689/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:281678/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:277513/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:276274/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:273492/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:27033/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:268258/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:264379/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:261593/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:55267/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:96782/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:92326/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:86496/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:84200/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:81149/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:77045/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:72313/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:67152/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:65356/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:60401/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:58647/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:333/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:53930/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:51836/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:49065/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:44793/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:41360/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:37263/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:347745/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:343365/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:340517/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:260373/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:336851/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:14259/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:188365/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:185860/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:183416/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:179514/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:176400/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:172191/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:163180/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:161399/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:157811/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:149922/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:148456/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:190583/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:13794/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:135285/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:132664/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:130969/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:127566/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:125994/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:122448/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:119061/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:115019/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:113346/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:109097/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:223685/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:253722/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:252454/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:248886/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:245278/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:243369/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:2416/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:239483/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:234243/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:232541/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:230219/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:226632/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:100527/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:218570/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:213725/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:211819/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:20852/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:205432/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:197361/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:195821/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg > 1:194029/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt > 1:20095/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt > 1:95189/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTACCACCTTGTTCgggtg > 1:302772/1‑60 (MQ=25) ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTCAAACTTCCACcttgtt > 1:310322/1‑56 (MQ=21) | GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA > W3110S.gb/3498872‑3498933 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |