Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 3,498,922 G→C intergenic (+173/‑102) gldA → / → yijF glycerol dehydrogenase, NAD/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,498,9220GC99.8% 1015.5 / ‑7.5 495intergenic (+173/‑102)gldA/yijFglycerol dehydrogenase, NAD/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (494/0);  minor base A (1/0);  total (495/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 503 total aligned reads displayed.

GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA  >  W3110S.gb/3498872‑3498933
                                                  |           
ggCGTTCCCGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:241748/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACcttgtt        >  1:167532/1‑56 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:293433/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:329340/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:327477/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:325207/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:323079/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:320169/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:314633/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:310526/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:308243/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:30291/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:29970/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:295722/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:334351/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:286443/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:283797/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:281866/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:278124/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:276733/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:273658/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:271226/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:268886/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:266186/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:262710/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:100527/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:55267/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:96782/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:92326/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:86496/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:84200/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:81149/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:77045/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:72313/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:67152/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:65356/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:60401/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:58647/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:260636/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:53930/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:51836/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:49065/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:45019/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:41622/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:37566/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:348644/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:34391/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:3417/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:338313/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:336869/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:142797/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:183656/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:181853/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:177574/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:1726/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:163180/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:161399/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:157811/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:153265/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:150089/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:148674/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:146938/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:186471/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:137941/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:135726/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:132676/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:131163/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:127783/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:126269/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:119946/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:115019/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:113346/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:109097/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:223963/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:253098/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:251533/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:248018/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:245963/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:244005/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:240425/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:235208/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:23264/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:230526/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:226840/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:189138/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:219638/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:214062/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:211996/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:209188/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:206432/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:201172/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:199247/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:195921/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:194302/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:19141/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt   >  1:28994/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggt   >  1:95189/1‑60 (MQ=25)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTAGTTCgggtg  >  1:123358/1‑60 (MQ=21)
ggCGTTCACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTAGTTCgggtg  >  1:331181/1‑60 (MQ=21)
ggCGTTAACGCCGCATCCGGCACGCTGTGCCAGTAATCTGAAACTTCCACCTTGTTCgggtg  >  1:254819/1‑60 (MQ=21)
                                                  |           
GGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAA  >  W3110S.gb/3498872‑3498933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑