Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | W3110S.gb | 567,753 | C→T | S72S (AGC→AGT) | emrE → | multidrug resistance protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 567,753 | 0 | C | T | 99.8% | 1067.6 / NA | 565 | S72S (AGC→AGT) | emrE | multidrug resistance protein |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (564/0); minor base G (1/0); total (565/0) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
Only 100 of 565 total aligned reads displayed.
TGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGC > W3110S.gb/567746‑567807 | ttaTTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:201842/3‑62 (MQ=16) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACTACTGGACCTGCCAGCTATCATAGGc > 1:188296/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGAc > 1:288185/1‑44 (MQ=12) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTg > 1:233702/1‑47 (MQ=16) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTAt > 1:264431/1‑55 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATcata > 1:159/1‑59 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:95009/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:320837/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:335027/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:221037/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:223529/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:160983/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:15347/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:109331/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:25038/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:291729/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:66866/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:72576/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:78763/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAgg > 1:266630/1‑61 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAg > 1:230434/1‑60 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:305737/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:315210/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:276525/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:313397/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:259599/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:309238/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:307677/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:278716/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:294671/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:302108/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:300998/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:298595/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:295772/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:47976/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:92179/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:9017/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:85667/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:76222/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:68561/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:65313/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:62393/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:59435/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:54086/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:51947/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:31619/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:45094/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:40899/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:38058/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:347914/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:345404/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:342714/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:341063/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:338306/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:329152/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:140308/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:181900/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:178710/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:176137/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:170984/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:167073/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:163582/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:156271/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:151877/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:148459/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:146406/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:144155/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:184955/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:137977/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:134623/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:132/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:128087/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:125226/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:121631/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:11968/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:115916/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:113546/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:103211/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:236211/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:269109/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:265772/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:100801/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:254406/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:246033/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:244105/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:241689/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:239703/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:272057/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:226151/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:218910/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:216203/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:213173/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:209110/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:196305/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:194413/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:190324/1‑62 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTTTTTGGTCAACGACAGGACCTGCCAGCAATcata > 1:284688/1‑59 (MQ=255) tGATTAGTTTGCTTTCCTGGGGATTATTTGGTCAACGACTGGACCTgcc > 1:20638/1‑49 (MQ=12) tGATTAGTTTGCTTTCATGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:28071/1‑62 (MQ=255) tGATTAGGTTGCTTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGc > 1:324183/1‑62 (MQ=255) | TGATTAGCTTACTGTCATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGC > W3110S.gb/567746‑567807 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |