Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,041,433 T→C Y225Y (TAT→TAC)  livJ → leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,041,4330TC99.8% 951.3 / NA 529Y225Y (TAT→TAC) livJleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (0/528);  minor base G (0/1);  total (0/529)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 529 total aligned reads displayed.

GGCGCGTCTGAAAAAAGAGAATATCGACTTCGTTTACTACGGCGGTTATCACCCGGAAATGG  >  W3110S.gb/4041385‑4041446
                                                |             
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACTGCGGTTACCACCCGGAAATgg  <  1:72215/62‑1 (MQ=17)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCTCCCGGAAATgg  <  1:6396/62‑1 (MQ=17)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCTGAAATgg  <  1:52876/62‑1 (MQ=17)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:324741/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:338338/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:324127/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:320846/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:317676/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:313806/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:312482/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:305070/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:30003/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:296542/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:294170/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:290562/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:288182/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:285707/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:281966/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:279905/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:272395/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:267354/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:264033/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:257553/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:254417/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:100707/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:248649/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:63070/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:9694/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:95014/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:92575/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:87659/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:86525/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:85041/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:81549/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:78385/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:76362/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:73853/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:69374/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:329165/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:58476/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:55008/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:51532/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:44767/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:42555/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:39050/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:347935/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:340793/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:251423/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:333844/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:138854/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:174240/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:169050/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:167661/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:165649/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:161889/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:157384/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:156001/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:152392/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:148560/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:147320/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:142508/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:139721/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:177838/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:135661/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:131626/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:129247/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:126117/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:122591/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:116527/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:112869/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:111789/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:108670/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:104995/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:1032/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:207025/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:235126/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:230976/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:228130/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:239732/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:224191/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:220380/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:218178/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:216275/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:212036/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:246197/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:20934/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:20439/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:202082/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:24088/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:19545/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:193697/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:191455/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:190042/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:181846/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:17975/62‑1 (MQ=255)
ggCGCGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:245161/62‑1 (MQ=255)
 gcgcGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:67500/61‑1 (MQ=255)
 gcgcGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:276851/61‑1 (MQ=255)
 gcgcGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:197334/61‑1 (MQ=255)
 gcgcGTCTGAAGAAAGAGAATATCGACTTCGTTTACTACGGCGGTTACCACCCGGAAATgg  <  1:331980/61‑1 (MQ=255)
                                                |             
GGCGCGTCTGAAAAAAGAGAATATCGACTTCGTTTACTACGGCGGTTATCACCCGGAAATGG  >  W3110S.gb/4041385‑4041446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑