Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,180,554 T→C Q16Q (CAA→CAG)  gspF ← general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,180,5540TC99.9% 1212.2 / NA 672Q16Q (CAA→CAG) gspFgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (0/671);  minor base A (0/1);  total (0/672)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 673 total aligned reads displayed.

GTTCATCGTTAGCATCAATGATCCCTTGCAATTTTTGACCATCCTGGGTCATGGCGCGATAGC  >  W3110S.gb/4180529‑4180591
                         |                                     
 tttaTCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:272976/59‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACTATCCTGGGTCATGGCgcgatagc  <  1:136400/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTTGGTCATGGCgcgatagc  <  1:28881/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:300157/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:335576/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:332026/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:329961/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:325706/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:323258/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:320213/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:318110/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:315506/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:310998/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:307134/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:305343/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:301973/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:337808/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:296247/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:293198/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:286334/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:282663/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:279311/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:277759/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:27617/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:271393/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:62158/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:10024/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:93691/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:92165/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:9034/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:85211/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:8215/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:80428/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:75266/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:69610/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:67704/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:65697/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:96824/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:61483/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:57942/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:52332/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:49812/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:46731/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:42591/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:36183/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:345370/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:342716/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:340519/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:150205/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:186921/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:18238/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:269715/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:177658/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:174563/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:171551/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:165251/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:164057/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:159067/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:155395/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:152963/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:180564/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:148524/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:144673/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:14053/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:13133/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:127008/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:125712/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:118682/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:115471/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:109986/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:105583/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:10312/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:195690/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:26806/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:263714/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:258382/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:256994/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:24643/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:244080/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:24227/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:236795/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:234020/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:227249/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:223549/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:219943/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:215985/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:213762/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:210318/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:206900/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:200510/62‑1 (MQ=255)
 ttCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:19225/62‑1 (MQ=255)
 ttCACCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:2394/62‑1 (MQ=255)
 ttCAACGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:251121/62‑1 (MQ=255)
  tCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:54939/61‑1 (MQ=255)
  tCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:232076/61‑1 (MQ=255)
  tCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:31350/61‑1 (MQ=255)
  tCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:3996/61‑1 (MQ=255)
  tCATCGTTAGCATCAATGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:87282/61‑1 (MQ=255)
                  tGATCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:161479/45‑1 (MQ=255)
                     tCCCCTGCAATTTTTGACCATCCTGGGTCATGGCgcgatagc  <  1:71099/42‑1 (MQ=255)
                         |                                     
GTTCATCGTTAGCATCAATGATCCCTTGCAATTTTTGACCATCCTGGGTCATGGCGCGATAGC  >  W3110S.gb/4180529‑4180591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑