Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	222,403	G→A	R81R (CGC→CGT)	metN ←	DL‑methionine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	222,403	0	G	A	100.0%	157.2 / NA	78	R81R (CGC→CGT)	metN	DL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/78);  total (0/78)

ACGCGAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGGCGAGCTTTGGTC  >  W3110S.gb/222355‑222415                                                 |             aCGCGAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:46622/61‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTTGCGACGAGCTTTGGTc  <  1:6049/60‑1 (MQ=39)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTTGTc  <  1:44421/60‑1 (MQ=38)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:44228/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:46524/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:46407/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:46313/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:45789/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:44982/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:44744/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:44244/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:46963/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:44219/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:4364/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:43148/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:4228/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:42175/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:41268/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:40611/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:40493/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:50648/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:7795/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:54164/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:53305/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:5288/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:5279/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:52584/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:51100/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:50954/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:14047/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:50642/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:50618/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:50122/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:48629/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:48305/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:4821/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:47945/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:47619/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:21704/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:25199/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:25022/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:22817/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:22665/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:22473/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:2247/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:22407/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:22150/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:38635/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:19025/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:18968/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:18062/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:17128/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:15907/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:15375/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:15314/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:32662/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:38247/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:38211/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:37318/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:37219/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:39576/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:35850/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:35563/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:34946/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:33054/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:31836/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:30402/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:29654/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:29633/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:29160/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:2910/60‑1 (MQ=255)  cgcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:28411/60‑1 (MQ=255)  cgcaAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:24187/60‑1 (MQ=38)   gcgAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:13100/59‑1 (MQ=255)      aaGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:8664/56‑1 (MQ=255)         agagCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:28217/53‑1 (MQ=255)                 ttAAAATGCTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:49099/45‑1 (MQ=255)                         cTGGAAAATCATACCAATCTGGCGACGAGCTTTGGTc  <  1:18363/37‑1 (MQ=255)                                                 |             ACGCGAAGAGAGCAGGTTAAAATGCTGGAAAATCATACCAATCTGGCGGCGAGCTTTGGTC  >  W3110S.gb/222355‑222415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑