Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,178,865	C→T	R264Q (CGG→CAG)	gatZ ←	D‑tagatose 1,6‑bisphosphate aldolase 2, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,178,865	0	C	T	100.0%	100.4 / NA	51	R264Q (CGG→CAG)	gatZ	D‑tagatose 1,6‑bisphosphate aldolase 2, subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/0);  total (51/0)

ATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTG  >  W3110S.gb/2178824‑2178872                                          |        aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTc    >  1:10696/3‑47 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCt   >  1:52875/3‑48 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:42976/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:32049/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:32084/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:32364/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:32437/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:36945/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:37587/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:37728/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:39050/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:40168/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:40788/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:42756/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:28812/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:42990/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:43643/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:43905/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:4407/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:46204/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:50103/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:50263/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:51902/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:53253/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:7966/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:8890/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:20440/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:10917/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:11505/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:14038/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:14405/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:1447/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:14546/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:14595/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:1608/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:16610/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:17766/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:18914/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:19272/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:29907/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:21325/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:21434/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:21582/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:22295/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:22695/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:24304/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:24726/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:24880/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:25241/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:26244/3‑49 (MQ=255) aaatTGCAAAATGATCGCGAACTAATTCCCGATAGGCCGTCTGAGTCTg  >  1:27919/3‑49 (MQ=255)                                          |        ATATTGCAAAGTGATCGCGGACTAATTCCCAATAAGCCGTCCGGGTCTG  >  W3110S.gb/2178824‑2178872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑