Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,873,023	T→C	P351P (CCA→CCG)	cysN ←	sulfate adenylyltransferase, subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,873,023	0	T	C	100.0%	29.8 / NA	17	P351P (CCA→CCG)	cysN	sulfate adenylyltransferase, subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/17);  total (0/17)

GTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCT  >  W3110S.gb/2873011‑2873072             |                                                  gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:39917/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:9827/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:6956/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:6738/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:5668/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:52812/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:46656/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:45732/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:41116/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:10702/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:3701/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:36469/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:25466/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:2212/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:20915/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:16011/62‑1 (MQ=255) gTAACTCTGGCCCGGGGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGctct  <  1:12450/62‑1 (MQ=255)             |                                                  GTAACTCTGCCCTGGAGAAAGCGGCTGTTCCGCCATCCATACCACATCCACCGACGCGCTCT  >  W3110S.gb/2873011‑2873072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑