Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,218,225	C→T	L35L (CTG→TTG)	ygjG →	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,218,225	0	C	T	100.0%	55.2 / NA	32	L35L (CTG→TTG)	ygjG	putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/32);  total (0/32)

AGCGAACGCTGGATCATGAGGAGATGAAAGCACTT  >  W3110S.gb/3218217‑3218251         |                           aGCGAACGTTTGATCATGAGGAGATGAAAGCACtt  <  1:54086/35‑1 (MQ=37) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:36705/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:9420/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:9136/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:6088/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:51118/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:49788/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:48835/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:45571/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:44381/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:43993/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:42967/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:41557/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:40841/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:3872/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:37930/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:10209/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:29825/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:2629/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:25390/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:24657/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:24394/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:22327/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:21617/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:20828/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:1923/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:17252/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:14147/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:12533/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:12397/35‑1 (MQ=255) aGCGAACGTTGGATCATGAGGAGATGAAAGCACtt  <  1:1180/35‑1 (MQ=255) aGCGAACGTGGGATCATGAGGAGATGAAAGCACtt  <  1:16811/35‑1 (MQ=37)         |                           AGCGAACGCTGGATCATGAGGAGATGAAAGCACTT  >  W3110S.gb/3218217‑3218251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑