Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,823,013	A→G	D243D (GAT→GAC)	yicC ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,823,013	0	A	G	100.0%	162.8 / NA	79	D243D (GAT→GAC)	yicC	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (79/0);  total (79/0)

CATAAAATCCAGACGACGACCAACCGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGA  >  W3110S.gb/3823007‑3823068       |                                                        catAAAGTCCAGACTACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:13309/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTttcttc                        >  1:21051/1‑40 (MQ=37) catAAAGTCCAGACGACGACCAACGGCTTCTTTTttcttaa                       >  1:21357/1‑39 (MQ=25) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGtctc       >  1:31899/1‑57 (MQ=39) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTg   >  1:54002/1‑61 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTg   >  1:45516/1‑61 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTg   >  1:49385/1‑61 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTg   >  1:25365/1‑61 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTg   >  1:24257/1‑61 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:40812/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:33926/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:40736/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:40310/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:3997/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:3946/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:38566/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:37994/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:37959/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:37948/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:37456/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:36538/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:34773/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:34185/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:32048/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:49247/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:9981/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:9614/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:8520/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:52781/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:51860/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:50880/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:49810/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:49324/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:40924/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:49090/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:49008/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:48704/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:48315/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:47652/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:43960/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:43246/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:13409/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:33604/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:18617/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:17940/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:17883/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:17777/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:16210/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:1564/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:14215/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:13602/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:20857/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:13391/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:11632/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:11582/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:11446/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:11342/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:11069/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:10612/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:10503/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:2191/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:33022/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:10110/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:31682/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:31671/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:30534/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:2897/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:26418/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:24751/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:24606/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:23745/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:23429/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:23341/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:22395/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:22171/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGa  >  1:21468/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCATTGa  >  1:33658/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTCTTTTTTCTTCAGAATGATGTAGGTCTCTTTGa  >  1:37211/1‑62 (MQ=255) catAAAGTCCAGACGACGACCAACGGCTTATTTTttcttc                        >  1:6967/1‑40 (MQ=255)       |                                                        CATAAAATCCAGACGACGACCAACCGCTTCTTTTTTCTTCAGAATGTTGTAGGTCTCTTTGA  >  W3110S.gb/3823007‑3823068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑