Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,853,262	C→T	L106L (CTG→CTA)	envC ←	protease with a role in cell division

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,853,262	0	C	T	100.0%	112.4 / NA	57	L106L (CTG→CTA)	envC	protease with a role in cell division
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (57/0);  total (57/0)

CTCCTGGGCGGCTTTTTGCTGCTCCAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTT  >  W3110S.gb/3853238‑3853299                         |                                      cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGAc                        >  1:5022/1‑40 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTtcatctcat           >  1:53712/1‑53 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTtcatctca            >  1:14536/1‑52 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAAt       >  1:3189/1‑57 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:40895/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:40430/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:41959/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:41989/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:42371/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:43314/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:43681/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:44758/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:45190/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:46381/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:46658/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:46743/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:47411/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:49684/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:50870/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:51965/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:53996/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:54231/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:559/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:763/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:7661/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:8016/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:8707/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:8945/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:900/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:25406/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:12693/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:16965/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:17555/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:18086/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:18746/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:20002/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:21013/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:21313/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:2164/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:21686/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:23704/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:23812/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:40717/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:25978/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:28777/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:30036/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:32309/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:32356/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:32460/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:33161/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:3524/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:35938/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:36208/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:36932/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:37631/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGtt  >  1:1267/1‑62 (MQ=255) cTCCTGGGCGGCTTTTTGCTGCTCTAGTTTGGCAATCGACGCGTTCATCTCATCAATATGtt  >  1:6538/1‑62 (MQ=255)                         |                                      CTCCTGGGCGGCTTTTTGCTGCTCCAGTTTGGCAATCGACGCGTTCATCTCATCAATCTGTT  >  W3110S.gb/3853238‑3853299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑