Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,927,638	T→C	S52S (AGT→AGC)	yhjY →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,927,638	0	T	C	100.0%	117.6 / NA	59	S52S (AGT→AGC)	yhjY	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (59/0);  total (59/0)

GAGCGTTACACCTGGGATAGTGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTA  >  W3110S.gb/3927618‑3927678                     |                                         gagaGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:17099/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATAtt               >  1:48144/1‑48 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGt   >  1:45507/1‑60 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:46700/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:34758/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:36820/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:38056/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:38069/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:3935/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:40570/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:43972/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:44346/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:4441/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:44437/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:44991/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:46119/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:46543/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:32226/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:47077/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:47741/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:48054/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:48549/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:50564/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:53072/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:53763/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:53886/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:5776/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:584/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:6229/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:6983/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:21343/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:12650/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:13335/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:14002/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:1415/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:14986/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:15448/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:15478/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:17488/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:17540/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:17609/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:18173/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:18593/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:19283/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:21300/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:33588/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:2150/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:2185/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:21891/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:2283/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:23044/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:24663/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:24714/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:28566/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:30041/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:30481/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:30914/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:31551/1‑61 (MQ=255) gagCGTTACACCTGGGATAGCGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTa  >  1:11891/1‑61 (MQ=255)                     |                                         GAGCGTTACACCTGGGATAGTGATCATCAACCTGATTACAACGATATTTTGTCGCAACGTA  >  W3110S.gb/3927618‑3927678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑