Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,937,153	A→G	L195L (CTA→CTG)	dppD →	dipeptide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,937,153	0	A	G	100.0%	120.9 / NA	61	L195L (CTA→CTG)	dppD	dipeptide transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/61);  total (0/61)

TGACCATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCG  >  W3110S.gb/3937125‑3937186                             |                                  tGACCATTCAGGCGCAAATTATCGAACTGTTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:4988/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACCGCAGAAAGAGAACATGgcg  <  1:2847/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATggtg  <  1:47248/62‑3 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:49633/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:39640/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:40065/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:40163/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:40542/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:41157/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:41432/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:4251/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:43424/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:44094/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:44271/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:4545/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:476/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:49517/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:38167/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:50784/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:51884/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:52004/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:52263/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:52765/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:52789/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:52843/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:52866/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:54515/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:6661/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:78/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:9126/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:9670/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:27790/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:11333/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:11612/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:11774/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:12536/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:16459/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:17795/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:18545/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:20420/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:20687/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:22735/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:24958/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:25528/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:26687/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:38236/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:28240/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:28470/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:30112/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:30136/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:30784/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:31004/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:31192/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:31890/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:33107/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:33351/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:33457/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:35416/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:37642/62‑1 (MQ=255) tGACCATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGgcg  <  1:10575/62‑1 (MQ=255)  gagcATTCAGGCGCAAATTATCGATATGCTGCTGTAGCTACTGCAGAAAGAGAACATGgcg  <  1:22050/58‑1 (MQ=255)                             |                                  TGACCATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCG  >  W3110S.gb/3937125‑3937186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑