Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,084,997	A→G	R779R (CGT→CGC)	malT ←	DNA‑binding transcriptional activator, maltotriose‑ATP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,084,997	0	A	G	100.0%	58.3 / NA	31	R779R (CGT→CGC)	malT	DNA‑binding transcriptional activator, maltotriose‑ATP‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/31);  total (0/31)

CAGCTGACGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGGCTGATA  >  W3110S.gb/4084991‑4085045       |                                                 aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAGTGACTGATa  <  1:35566/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGAt   <  1:47097/53‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:9050/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:10643/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:53974/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:53838/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:51279/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:50597/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:50450/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:48521/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:47954/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:46504/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:46476/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:43053/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:42333/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:39560/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:38042/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:32638/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:32535/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:30575/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:24511/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:20550/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:20373/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:2034/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:17762/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:17473/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:16068/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:15966/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:11362/54‑1 (MQ=255) aaGCTGGCGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:10694/54‑1 (MQ=255) aaGCTGGCCCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGACTGATa  <  1:33562/54‑1 (MQ=255)       |                                                 CAGCTGACGCAGTTGTTGCGCCATCGCTTCGCCTTCGATGACAAAATGGCTGATA  >  W3110S.gb/4084991‑4085045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑