Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,405,560 T→C A69A (GCT→GCC)  hflX → predicted GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,405,5600TC100.0% 250.3 / NA 120A69A (GCT→GCC) hflXpredicted GTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/120);  total (0/120)

Only 100 of 120 total aligned reads displayed.

ATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCC  >  W3110S.gb/4405549‑4405610
           |                                                  
aTTGCGGAAGCCGTAAAAGCGACGGTAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:49157/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:3780/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:44107/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:43349/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:42489/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:42272/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:41558/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:38578/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:37967/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:44312/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:11219/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:34753/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:33765/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:3286/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:32334/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:32225/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:32191/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:44308/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:37038/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:46360/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:47873/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:48139/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:48545/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:50060/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:50216/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:51063/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:51934/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:52666/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:6850/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:7223/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:8638/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:9205/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:9804/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:28659/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:17529/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:17221/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:16435/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:23970/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:21272/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:24870/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:25088/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:21227/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:14300/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:1388/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:11581/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:2102/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:11597/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:1293/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:28367/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCATTCTTTTTGACCATGCCCTGAGccc  <  1:1816/62‑1 (MQ=255)
aTTGCGGAAGCCGTAAAAGCGACGGGAGCTGCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:51500/62‑1 (MQ=255)
aTTGCGGAACCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:44197/62‑1 (MQ=255)
 ttccGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:37973/58‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGGCCATGCCCTGAGccc  <  1:19725/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:46397/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:45930/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:47165/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:47324/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:11363/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:45767/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:48720/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:15837/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:50410/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:15436/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:15378/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:51961/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:544/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:6908/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:8711/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:9409/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:24604/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:2938/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:30380/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:30941/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:30975/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:31768/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:28526/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:27177/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:25461/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:33276/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:35539/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:44341/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:24564/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:21193/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:43770/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:43421/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:21735/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:2249/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:41964/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:38005/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:23126/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGGTCTTTTTGACCATGCCCTGAGccc  <  1:36302/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGGCGTTCTTTTTGACCATGCCCTGAGccc  <  1:6747/61‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACGGGAGC‑TCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:31802/60‑1 (MQ=255)
 ttGCGGAAGCCGTAAAAGCGACAGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:4102/61‑1 (MQ=255)
  tGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:33363/60‑1 (MQ=255)
  tGCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:23262/60‑1 (MQ=255)
   gCGGAAGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:50461/59‑1 (MQ=255)
       aaGCCGTAAAAGCGACGGGAGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGccc  <  1:16707/55‑1 (MQ=255)
        aGCCGTAAAAGCGACGGGAGCTTCGGGCGTTCTTTTTGACCATGCCCTGAGccc  <  1:40296/54‑1 (MQ=255)
           |                                                  
ATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCC  >  W3110S.gb/4405549‑4405610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑