Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 654214–655411 655412 2–1199 44 [0] [0] 41 [dcuC]–[dcuC] [dcuC],insH,[dcuC]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 6542130 (0.000)33 (0.960) 23/90 NT 100% coding (70/477 nt) dcuC anaerobic C4‑dicarboxylate transport
?W3110S.gb 655413 = 0 (0.000)coding (978/1092 nt) dcuC anaerobic C4‑dicarboxylate transport

TCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/654141‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCA  >  W3110S.gb/655413‑655474
                                                                                                                                       
TCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTA                                                                  <  1:524727/71‑1
TCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTA                                                                  <  1:726559/71‑1
  AGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG                                                                >  1:1181549/1‑71
  AGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG                                                                >  1:578326/1‑71
  AGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG                                                                >  1:2020200/1‑71
    GATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCC                                                              <  1:2252138/71‑1
        ATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTG                                                          <  1:1699564/71‑1
           CTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCC                                                             <  1:2751171/65‑1
           CTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCC                                                             <  1:465455/65‑1
            TCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                       >  1:839348/1‑70
             CGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCC                                                             >  1:91317/1‑63
               CCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAA                                                                 <  1:192246/57‑1
                CGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                      <  1:193454/67‑1
                    CCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCC                                              <  1:2087898/71‑1
                    CCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCC                                               >  1:627614/1‑70
                      AAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                      <  1:1392611/61‑1
                       AACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                      <  1:2422928/60‑1
                       AACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                   >  1:2071517/1‑47
                       AACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                   >  1:3090824/1‑47
                        ACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCC                                                             >  1:2312775/1‑52
                        ACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCC                                                             >  1:3612951/1‑52
                            GGTACCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAA                                      <  1:405337/71‑1
                               AGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCAAGCGGCAAC                                     >  1:2376369/1‑69
                               AGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAA                                                                 >  1:1662555/1‑41
                                GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAG                                  <  1:682558/71‑1
                                    TAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGC                              <  1:2644979/71‑1
                                    TAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGC                              <  1:2585283/71‑1
                                    TAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAG                               >  1:2381790/1‑70
                                     AGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCA                             >  1:1554373/1‑71
                                      GAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCACCCAGCAGCAT                            >  1:1910889/1‑71
                                           CAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCA                       >  1:2010386/1‑71
                                            AGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCAC                      >  1:3561748/1‑71
                                            AGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAA                                      >  1:229990/1‑55
                                              ACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                      <  1:3162529/37‑1
                                              ACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                      <  1:1432412/37‑1
                                              ACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                       <  1:2019237/36‑1
                                               CTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCACCCAGCAGCATCACCACGT                    >  1:919947/1‑70
                                               CTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                    >  1:2804155/1‑70
                                               CTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                    >  1:915723/1‑70
                                                TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAA                                                    >  1:622857/1‑37
                                                TTTGAATAAAGCCGATGGTCCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCA                             <  1:3593935/60‑1
                                                     ATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGC                                 <  1:3191168/51‑1
                                                      TAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAAC            <  1:2308108/71‑1
                                                      TAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAAC            <  1:2416123/71‑1
                                                        AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACG           >  1:2603286/1‑70
                                                        AAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACG           >  1:920423/1‑70
                                                              ATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGC    <  1:3355531/71‑1
                                                              ATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCA               <  1:3441051/60‑1
                                                                GGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCA  >  1:1169632/1‑71
                                                                      AAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCAT        >  1:557153/1‑59
                                                                                                                                       
TCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/654141‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCA  >  W3110S.gb/655413‑655474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.